Arthrobacter phage Mudpuppy
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Detailed Information for Phage Mudpuppy
Discovery Information
Isolation HostArthrobacter globiformis NRRL B-2880
Found ByJessica Hursti
Year Found2021
Location FoundOttawa, Canada
Finding InstitutionUniversity of Ottawa
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates45.3624 N, 75.9886 W Map
Discovery NotesThe phage was isolated from a soil sample taken from the bank of a pond in rural West Ottawa. The phage was extracted using the direct isolation method. The soil sample was dark, dense, and wet.
Naming NotesThis phage was named after a North American aquatic salamander. This amphibian was regularly caught in this pond at my grandmother's house by my siblings and I growing up, leading to the affectionate name "Mudpuppy pond".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 13, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1582
Genome length (bp)43824
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGAAGGGGCAT
GC Content67.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAZ
SubclusterAZ1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Ibadan
Annotation StatusIn GenBank
Plaque NotesMay be fully lytic.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Mudpuppy_1
(terminase, small subunit)

Mudpuppy_2
(terminase, large subunit)

Mudpuppy_3
(portal protein)

Mudpuppy_4
(capsid maturation protease and VIP2-like ADP-ribosyltransferase toxin)

Mudpuppy_5

Mudpuppy_6
(scaffolding protein)

Mudpuppy_7
(major capsid protein)

Mudpuppy_8
(head-to-tail adaptor)

Mudpuppy_9

Mudpuppy_10
(head-to-tail stopper)

Mudpuppy_11

Mudpuppy_12
(tail terminator)

Mudpuppy_13
(major tail protein)

Mudpuppy_14
(tail assembly chaperone)

Mudpuppy_15
(tail assembly chaperone)

Mudpuppy_16
(tape measure protein)

Mudpuppy_17
(minor tail protein)

Mudpuppy_18
(minor tail protein)

Mudpuppy_19
(minor tail protein)

Mudpuppy_20
(minor tail protein)

Mudpuppy_21

Mudpuppy_22

Mudpuppy_23

Mudpuppy_24
(deoxynucleoside monophosphate kinase)

Mudpuppy_25

Mudpuppy_26
(exonuclease)

Mudpuppy_27
(nucleoside deoxyribosyltransferase)

Mudpuppy_28
(LAGLIDADG endonuclease)

Mudpuppy_29
(recombinational directionality factor)

Mudpuppy_30

Mudpuppy_31

Mudpuppy_32
(NrdH- like glutaredoxin)

Mudpuppy_33

Mudpuppy_34
(Holliday junction resolvase)

Mudpuppy_35
(DNA methyltransferase)

Mudpuppy_36
(DNA primase/helicase)

Mudpuppy_37

Mudpuppy_38
(DNA Polymerase I)

Mudpuppy_39
(DNA ligase)

Mudpuppy_40

Mudpuppy_41
(DNA binding protein)

Mudpuppy_42
(DNA binding protein)

Mudpuppy_43
(SprT-like protease)

Mudpuppy_44

Mudpuppy_45

Mudpuppy_46
(serine integrase)

Mudpuppy_47

Mudpuppy_48
(RNA binding protein)

Mudpuppy_49

Mudpuppy_50

Mudpuppy_51

Mudpuppy_52

Mudpuppy_53

Mudpuppy_54

Mudpuppy_55
(endolysin)

Mudpuppy_56

Mudpuppy_57

Mudpuppy_58

Mudpuppy_59

Mudpuppy_60

Mudpuppy_61

Mudpuppy_62

Mudpuppy_63

Mudpuppy_64

Mudpuppy_65

Mudpuppy_66

Mudpuppy_67

Mudpuppy_68
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPQ184811
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 133
Pitt Freezer Box Grid# D10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload