Detailed Information for Phage Mudpuppy |
Discovery Information |
Isolation Host | Arthrobacter globiformis NRRL B-2880 |
Found By | Jessica Hursti |
Year Found | 2021 |
Location Found | Ottawa, Canada |
Finding Institution | University of Ottawa |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | Not entered |
GPS Coordinates | 45.3624 N, 75.9886 W Map |
Discovery Notes | The phage was isolated from a soil sample taken from the bank of a pond in rural West Ottawa. The phage was extracted using the direct isolation method. The soil sample was dark, dense, and wet. |
Naming Notes | This phage was named after a North American aquatic salamander. This amphibian was regularly caught in this pond at my grandmother's house by my siblings and I growing up, leading to the affectionate name "Mudpuppy pond". |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Apr 13, 2023 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 1582 |
Genome length (bp) | 43824 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | CGAAGGGGCAT |
GC Content | 67.6% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | AZ |
Subcluster | AZ1 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | University of Ibadan |
Annotation Status | In GenBank |
Plaque Notes | May be fully lytic. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | PQ184811 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
133 |
Pitt Freezer Box Grid# |
D10 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |