Mycobacterium phage Creamsicle
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| Detailed Information for Phage Creamsicle | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Greta Hodges, Toulin Kusaibati |
| Year Found | 2023 |
| Location Found | Spokane, WA United States |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | Unavailable |
| Discovery Notes | September 29, 2023 There are spots present in everyone on our plates from our last time testing and diluting our phage. The top agar had started to solidify so our samples are a bit hard to read but you can tell that phage are present. Results (October 2, 2023): Plaques were present in our 10^-5 and 10^-6 plates. Webbing was present in our 10^-4 and a little less in our 10^-3 plate sample. Results (October 13, 2023): Our initial titer was 3.3 10^5 pfu/mL Our titers came out weird and not how they were supposed to. We suspect our top agar may have started to solidify before adding it to the plate and that would cause the titers to not create spots. Our 10^5 and 10^6 have potential. Results (October 18, 2023): We were supposed to have plaques and webbed plates but we did not. Since there were no plaques something must have gone wrong with our original titers. Our lysate was done on plaques. November 10th Our TEM results: Total length: 184.234 Tail length: 124.940 Head perimeter: 204.612 Head surface area: 3190.331 Results (November 3, 2023): Phage are present on our plates. New titter is 3.0 x 10^9 pfu/mL. Our lysate plate also has phage present. November 3rd Tested our DNA 5.47mL DNA sample |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 189 |
| Pitt Freezer Box Grid# | E6 |
| Available Files | |
| EM Picture | Download |