Gordonia phage CurlyShirley
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| Detailed Information for Phage CurlyShirley | |
| Discovery Information | |
| Isolation Host | Gordonia terrae CAG3 |
| Found By | Alex Niemann and Jazmin Graham |
| Year Found | 2024 |
| Location Found | Manhattan, KS United States |
| Finding Institution | Kansas State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 20°C |
| GPS Coordinates | 39.1141 N, 96.3555 W Map |
| Discovery Notes | Phage was discovered on September 17, 2024, at 7:55 a.m. in damp soil outside my apartment in a garden. The sample was collected using gloves and a ziplock bag. There had been rain at this location the night prior. When running a host range assay there was a faint plaque present at 10^-1 with Gordonia lacunae and a very strong plaque present at 10^-1 with Gordonia rubripertincta. These results indicate that the phage was postive for both bacteria. Conducted a sensitivity assay test on phage RanserRd, resulting with a positive result. CurlyShirley had colonies up to 10^6 that were positive with this test. |
| Naming Notes | When we examined the phage's morphology, we decided it looked semi-curly, which led Jazmin and Alex to decide that CurlyShirley would be a great name for it. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Did aspetic loop streaks and patch assay experiments to find the lysogeny of CurlyShirley. Streaks #4 & #5 were lysogeny positive colonies. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques looked irregular, and the boundaries were a little hazy; however, they were generally circular in shape. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |