Mycobacterium phage Daegal
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| Detailed Information for Phage Daegal | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Leani Oosthuizen |
| Year Found | 2017 |
| Location Found | Auckland, New Zealand |
| Finding Institution | Massey University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.734 S, 174.701 E Map |
| Discovery Notes | Found at the Massey University Community Garden Box, in wet, soft soil under straw. Adopted from another student in the program (Natasha Elliston-Boyes). Temperature on day of discovery was 21 degrees Celsius. |
| Naming Notes | Daegal is an Anglo-Saxon name that means "Dweller by the dark stream." This was apt as the phage was found in a garden near a forest stream. It also refers to this phage 'hiding' until it was isolated by phage-hunters. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 27, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3483 |
| Genome length (bp) | 54858 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 14 bases |
| Overhang Sequence | TAAGCCGCGCGGTA |
| GC Content | 67.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | Q |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Massey University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are very consistent in size and morphology. They are approximately 0.75 mm in diameter. Plaques are uniformly and completely clear in the centre with a circular in shape and clearly defined edges. Some evidence of lysogenic mesa was seen when very high titres (10^9) were spotted on bacterial lawn. The mesa in the centre of the clearance was not very opaque but definitely more cloudy than the usual completely clear lytic plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 87 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK494095 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.67 x 10^12 |
| Date of SEA Lysate Titering | Jul 5, 2017 |
| Pitt Freezer Box# | 164 |
| Pitt Freezer Box Grid# | A5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
