| Detailed Information for Phage Daegal | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Leani Oosthuizen |
| Year Found | 2017 |
| Location Found | Auckland, New Zealand |
| Finding Institution | Massey University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.734 S, 174.701 E Map |
| Discovery Notes | Found at the Massey University Community Garden Box, in wet, soft soil under straw. Adopted from another student in the program (Natasha Elliston-Boyes). Temperature on day of discovery was 21 degrees Celsius. |
| Naming Notes | Daegal is an Anglo-Saxon name that means "Dweller by the dark stream." This was apt as the phage was found in a garden near a forest stream. It also refers to this phage 'hiding' until it was isolated by phage-hunters. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 27, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3483 |
| Genome length (bp) | 54858 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 14 bases |
| Overhang Sequence | TAAGCCGCGCGGTA |
| GC Content | 67.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | Q |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Massey University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are very consistent in size and morphology. They are approximately 0.75 mm in diameter. Plaques are uniformly and completely clear in the centre with a circular in shape and clearly defined edges. Some evidence of lysogenic mesa was seen when very high titres (10^9) were spotted on bacterial lawn. The mesa in the centre of the clearance was not very opaque but definitely more cloudy than the usual completely clear lytic plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 87 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewDaegal_1 Daegal_2 Daegal_3 Daegal_4 Daegal_5 Daegal_6 Daegal_7 Daegal_8 Daegal_9 Daegal_10 Daegal_11 Daegal_12 Daegal_13 Daegal_14 Daegal_15 Daegal_16 Daegal_17 Daegal_18 Daegal_19 Daegal_20 Daegal_21 Daegal_22 Daegal_23 Daegal_24 Daegal_25 Daegal_26 Daegal_27 Daegal_28 Daegal_29 Daegal_30 Daegal_31 Daegal_32 Daegal_33 Daegal_34 Daegal_35 Daegal_36 Daegal_37 Daegal_38 Daegal_39 Daegal_40 Daegal_41 Daegal_42 Daegal_43 Daegal_44 Daegal_45 Daegal_46 Daegal_47 Daegal_48 Daegal_49 Daegal_50 Daegal_51 Daegal_52 Daegal_53 Daegal_54 Daegal_55 Daegal_56 Daegal_57 Daegal_58 Daegal_59 Daegal_60 Daegal_61 Daegal_62 Daegal_63 Daegal_64 Daegal_65 Daegal_66 Daegal_67 Daegal_68 Daegal_69 Daegal_70 Daegal_71 Daegal_72 Daegal_73 Daegal_74 Daegal_75 Daegal_76 Daegal_77 Daegal_78 Daegal_79 Daegal_80 Daegal_81 Daegal_82 Daegal_83 Daegal_84 Daegal_85 Daegal_86 Daegal_87 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK494095 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.67 x 10^12 |
| Date of SEA Lysate Titering | Jul 5, 2017 |
| Pitt Freezer Box# | 164 |
| Pitt Freezer Box Grid# | A5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
