| Detailed Information for Phage Darwin | |
| Discovery Information | |
| Isolation Host | Corynebacterium vitaeruminis NCIB 9291 |
| Found By | Dan Nguyen |
| Year Found | 2016 |
| Location Found | Birmingham, AL USA |
| Finding Institution | University of Alabama at Birmingham |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.44192024533113 N, 86.8248219 W Map |
| Discovery Notes | Discovered in the sewage of the Jefferson County Sewer Treatment Plant |
| Naming Notes | Name chosen by multiple reasons: *Association to Charles Darwin, the Father of Evolution. *Wordplay between pronunciation of Darwin and founder's name, Dan Nguyen. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1969 |
| Genome length (bp) | 67743 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCTACGGTGC |
| GC Content | 52.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EN |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to View |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques with an average diameter of 1 millimeter. Webbed plate occurring at 1/10000 dilution. |
| Number of Genes | 94 |
| Number of tRNAs | 5 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewDarwin_1 Darwin_2 Darwin_3 Darwin_4 Darwin_5 Darwin_6 Darwin_7 Darwin_8 Darwin_9 Darwin_10 Darwin_11 Darwin_12 Darwin_13 Darwin_14 Darwin_15 Darwin_16 Darwin_17 Darwin_18 Darwin_19 Darwin_20 Darwin_21 Darwin_22 Darwin_23 Darwin_24 Darwin_25 Darwin_26 Darwin_27 Darwin_28 Darwin_29 Darwin_31 Darwin_30 Darwin_32 Darwin_33 Darwin_34 Darwin_35 Darwin_36 Darwin_37 Darwin_38 Darwin_39 Darwin_40 Darwin_41 Darwin_42 Darwin_43 Darwin_44 Darwin_45 Darwin_46 Darwin_47 Darwin_48 Darwin_49 — tRNA Darwin_50 — tRNA Darwin_51 — tRNA Darwin_52 — tRNA Darwin_53 — tRNA Darwin_54 Darwin_55 Darwin_56 Darwin_57 Darwin_58 Darwin_59 Darwin_60 Darwin_61 Darwin_62 Darwin_63 Darwin_64 Darwin_65 Darwin_66 Darwin_67 Darwin_68 Darwin_69 Darwin_70 Darwin_71 Darwin_72 Darwin_73 Darwin_74 Darwin_75 Darwin_76 Darwin_77 Darwin_78 Darwin_79 Darwin_80 Darwin_81 Darwin_82 Darwin_83 Darwin_84 Darwin_85 Darwin_86 Darwin_87 Darwin_88 Darwin_89 Darwin_90 Darwin_91 Darwin_92 Darwin_93 Darwin_94 Darwin_95 Darwin_96 Darwin_97 Darwin_98 Darwin_99 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG198777 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.3*10^10 |
| Date of SEA Lysate Titering | Oct 12, 2016 |
| Pitt Freezer Box# | 32 |
| Pitt Freezer Box Grid# | C8 |
| Available Files | |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |