| Detailed Information for Phage DeepSoil15 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Chidera Nwude-Jacobs |
| Year Found | 2017 |
| Location Found | Washington, DC USA |
| Finding Institution | Howard University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.9177 N, 77.0212 W Map |
| Naming Notes | The name was derived from the location in which the phage was found, deep soil, and measured in close proximity to 15 ml in a tube. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 21, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Genome length (bp) | 53746 |
| Character of genome ends | Unknown |
| Overhang Length | 14 bases |
| Overhang Sequence | TAAGCCGCGCGGTA |
| GC Content | 67.4% |
| Sequencing Notes | About 12% of the reads from this sample were from an unrelated Cluster B3 phage, and thus it is likely that this was from a mixed lysate. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | Q |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of the Sciences (now St. Joseph's Univ) |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque is clear, with no visible texture on the surface. The plaques are not wide in margins. |
| Number of Genes | 86 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK919482 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.1x 10^10 PFU/mL |
| Pitt Freezer Box# |
52 |
| Pitt Freezer Box Grid# |
G6 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |