| Detailed Information for Phage Dorin |
| Discovery Information |
| Isolation Host | Rhodococcus equi NRRL B-16538 |
| Found By | Leilani Madrigal |
| Year Found | 2023 |
| Location Found | Grand Rapids, MI United States |
| Finding Institution | Calvin University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 42.928267 N, 85.587137 W Map |
| Discovery Notes | Soil was collected near a pond. The ground was damp, not very grassy. The soil was taken from the top layer of soil. The dirt itself was also damp, mulchy, and very spongy |
| Naming Notes | The origin of the name is Hebrew, which means gift. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 23, 2024 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 104 |
| Genome length (bp) | 136968 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 4720 bp |
| End Determination Notes | We ran PhageTerm on the Pastuer Institute's Galaxy server which showed and area of roughly 5 kb with ~2x the coverage of the rest of the genome. We inspected the ends of the region and found read buildups indicating the edges of a consistent terminal repeat. |
| GC Content | 48.0% |
| Sequencing Notes | Since this was the first of its cluster we'd seen, we oriented it so that the terminase and tapemeasure genes were in the forward direction. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CG |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | Calvin University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were consistent in circular shape and had very cloudy edges. They were about 0.5 mm in diameter. The plaques were very small, and not very abundant. |
| Morphotype | Siphoviridae |
| Number of Genes | 249 |
| Number of tRNAs | 33 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ114736 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
185 |
| Pitt Freezer Box Grid# |
A8 |
| Available Files |
| GenBank File for Phamerator | Download |