| Detailed Information for Phage Eastland | |
| Discovery Information | |
| Isolation Host | Streptomyces sanglieri UNT16F27A |
| Found By | Isaac Eastland |
| Year Found | 2019 |
| Location Found | Rowlett, TX United States of America |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 32.8881 N, 96.5752 W Map |
| Discovery Notes | Soil sample was taken from under the canopy of a large oak tree. The soil was mixed with acorns twigs and leaves. The sample was taken about one inch underground. The soil was dry with many sediments and had a consistent dark brown color. Temperature outside was 36.11 degrees Celsius, and dry |
| Naming Notes | The name of the phage came from my last name "Eastland". |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 26, 2021 |
| Sequencing Facility | University of North Texas |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 134 |
| Genome length (bp) | 37536 |
| Character of genome ends | Circularly Permuted |
| GC Content | 71.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BP |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | positive for lysogeny. The lawn fully covered the plate, there were no plaques or disturbances . |
| Other Cluster Members |
Click to View |
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | creates many tiny plaques ranging from 0.5mm to 1mm. cloudy with clear rings along the perimeter. |
| Morphotype | Siphoviridae |
| Number of Genes | 58 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewEastland_1 Eastland_2 Eastland_3 Eastland_4 Eastland_5 Eastland_6 Eastland_7 Eastland_8 Eastland_9 Eastland_10 Eastland_11 Eastland_12 Eastland_13 Eastland_14 Eastland_15 Eastland_16 Eastland_17 Eastland_18 Eastland_19 Eastland_20 Eastland_21 Eastland_22 Eastland_23 Eastland_24 Eastland_25 Eastland_26 Eastland_27 Eastland_28 Eastland_29 Eastland_30 Eastland_31 Eastland_32 Eastland_33 Eastland_34 Eastland_35 Eastland_36 Eastland_37 Eastland_38 Eastland_39 Eastland_40 Eastland_41 Eastland_42 Eastland_43 Eastland_44 Eastland_45 Eastland_46 Eastland_47 Eastland_48 Eastland_49 Eastland_50 Eastland_51 Eastland_52 Eastland_53 Eastland_54 Eastland_55 Eastland_56 Eastland_57 Eastland_58 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON392165 |
| Refseq Number | None yet |
| Published in Paper | Nath et al 2022, Microbiology Resource Announcements: Complete Genome Sequences of the Novel Cluster BP Phages Infecting Streptomyces sanglieri, AxeJC, Cumberbatch, Eastland, Eklok, HFrancette, Ignacio, Piccadilly, and Vondra |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 6.0*10^9 pfu/mL |
| Pitt Freezer Box# | 104 |
| Pitt Freezer Box Grid# | A11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
