| Detailed Information for Phage Eklok | |
| Discovery Information | |
| Isolation Host | Streptomyces sanglieri UNT16F27A |
| Found By | Erin Klokker |
| Year Found | 2019 |
| Location Found | Denton, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.211259 N, 97.14836 W Map |
| Discovery Notes | Collected soil at the Hurley Admin Building yard. It was 79°F, 9:30am. The soil was damp with a thickly rooted layer of plant on top. |
| Naming Notes | The phage is my nickname |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 30, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 6105 |
| Genome length (bp) | 37409 |
| Character of genome ends | Circularly Permuted |
| GC Content | 71.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BP |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | The phage is temperate and a lysogen was successfully isolated. |
| Other Cluster Members |
Click to View |
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Small (1.5mm) clear circular plaques |
| Number of Genes | 58 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewEklok_1 Eklok_2 Eklok_3 Eklok_4 Eklok_5 Eklok_6 Eklok_7 Eklok_8 Eklok_9 Eklok_10 Eklok_11 Eklok_12 Eklok_13 Eklok_14 Eklok_15 Eklok_16 Eklok_17 Eklok_18 Eklok_19 Eklok_20 Eklok_21 Eklok_22 Eklok_23 Eklok_24 Eklok_25 Eklok_26 Eklok_27 Eklok_28 Eklok_29 Eklok_30 Eklok_31 Eklok_32 Eklok_33 Eklok_34 Eklok_35 Eklok_36 Eklok_37 Eklok_38 Eklok_39 Eklok_40 Eklok_41 Eklok_42 Eklok_43 Eklok_44 Eklok_45 Eklok_46 Eklok_47 Eklok_48 Eklok_49 Eklok_50 Eklok_51 Eklok_52 Eklok_53 Eklok_54 Eklok_55 Eklok_56 Eklok_57 Eklok_58 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT521991 |
| Refseq Number | None yet |
| Published in Paper | Nath et al 2022, Microbiology Resource Announcements: Complete Genome Sequences of the Novel Cluster BP Phages Infecting Streptomyces sanglieri, AxeJC, Cumberbatch, Eastland, Eklok, HFrancette, Ignacio, Piccadilly, and Vondra |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.0x10^9pfu/mL |
| Pitt Freezer Box# | 104 |
| Pitt Freezer Box Grid# | A12 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
