| Detailed Information for Phage Evanesce | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Meghan Keleher |
| Year Found | 2012 |
| Location Found | Hartford, CT USA |
| Finding Institution | Trinity College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.75 N, 72.69 W Map |
| Discovery Notes | Soil collected in front of the Trinity College library on a patch of mostly dead grass. The soil was light to medium colors brown on surface but much darker once dug a little further down. Soil sample taken near a storing drain and sewer. No sprinklers nearby. |
| Naming Notes | Evanesce was chosen as it is a synonym for disappearing. When the EM picture was being taken of my phage, the tails would begin to disappear before the picture could be taken. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent |
| Approximate Shotgun Coverage | 1040 |
| Sanger Finishing Reactions | 10 |
| Genome length (bp) | 53746 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 14 bases |
| Overhang Sequence | TAAGCCGCGCGGTA |
| GC Content | 67.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | Q |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Phage is clear with a slight bull's eye. Faint turbid "halo". Mean diameter 24 hours post-infection was 0.8mm. 48 hours post- infection the phage grew in size to about 2.4mm and had a dark bull's eye in the middle with a lighter dark ring around the bull's eye and then a clear halo surrounding that. |
| Morphotype | Siphoviridae |
| Number of Genes | 85 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewEvanesce_1 Evanesce_2 Evanesce_3 Evanesce_4 Evanesce_5 Evanesce_6 Evanesce_7 Evanesce_8 Evanesce_9 Evanesce_10 Evanesce_11 Evanesce_12 Evanesce_13 Evanesce_14 Evanesce_15 Evanesce_16 Evanesce_17 Evanesce_18 Evanesce_19 Evanesce_20 Evanesce_21 Evanesce_22 Evanesce_23 Evanesce_24 Evanesce_25 Evanesce_26 Evanesce_27 Evanesce_28 Evanesce_29 Evanesce_30 Evanesce_31 Evanesce_32 Evanesce_33 Evanesce_34 Evanesce_35 Evanesce_36 Evanesce_37 Evanesce_38 Evanesce_39 Evanesce_40 Evanesce_41 Evanesce_42 Evanesce_43 Evanesce_44 Evanesce_45 Evanesce_46 Evanesce_47 Evanesce_48 Evanesce_49 Evanesce_50 Evanesce_51 Evanesce_52 Evanesce_53 Evanesce_54 Evanesce_55 Evanesce_56 Evanesce_57 Evanesce_58 Evanesce_59 Evanesce_60 Evanesce_61 Evanesce_62 Evanesce_63 Evanesce_64 Evanesce_65 Evanesce_66 Evanesce_67 Evanesce_68 Evanesce_69 Evanesce_70 Evanesce_71 Evanesce_72 Evanesce_73 Evanesce_74 Evanesce_75 Evanesce_76 Evanesce_77 Evanesce_78 Evanesce_79 Evanesce_80 Evanesce_81 Evanesce_82 Evanesce_83 Evanesce_84 Evanesce_85 |
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT454972 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 39 |
| Pitt Freezer Box Grid# | 71 |
| Available Files | |
| Restriction Digest Picture | Download |
| EM Picture | Download |
