| Detailed Information for Phage Ezurina | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis NRRL B-4425 |
| Found By | Ezana Ghedam |
| Year Found | 2025 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 45.251806 N, 75.300194 W Map |
| Discovery Notes | The sample came from a garden. The soil was dark, moist and soft, suggesting a rich environment for bacteria. Genomic DNA was sequenced on an ONT Minion using R10.4.1 chemistry. The sequencing reads assembled into two contigs. The longest contig was 16,844 bp, which showed ~60% coverage and 0 E-value alignments to the FR cluster phages Annabelle and Neuvillette. The second contig was shorter and likely represents an unresolved terminal region or an area of low read depth. |
| Naming Notes | Adopted bacteriophage from teaching assistant, name is a combination of both student and teaching assistant's name. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 822 |
| Genome length (bp) | 40606 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTACGGCAT |
| GC Content | 59.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FR |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | University of Ottawa |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | The plaques were round and clear. Only a few plaques were present on the 10⁻4 purified plate. |
| Morphotype | Siphoviridae |
| Number of Genes | 69 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewEzurina_1 Ezurina_2 Ezurina_3 Ezurina_4 Ezurina_5 Ezurina_6 Ezurina_7 Ezurina_8 Ezurina_9 Ezurina_10 Ezurina_11 Ezurina_12 Ezurina_13 Ezurina_14 Ezurina_15 Ezurina_16 Ezurina_17 Ezurina_18 Ezurina_19 Ezurina_20 Ezurina_21 Ezurina_22 Ezurina_23 Ezurina_24 Ezurina_25 Ezurina_26 Ezurina_27 Ezurina_28 Ezurina_29 Ezurina_30 Ezurina_31 Ezurina_32 Ezurina_33 Ezurina_34 Ezurina_35 Ezurina_36 Ezurina_37 Ezurina_38 Ezurina_39 Ezurina_40 Ezurina_41 Ezurina_42 Ezurina_43 Ezurina_44 Ezurina_45 Ezurina_46 Ezurina_47 Ezurina_48 Ezurina_49 Ezurina_50 Ezurina_51 Ezurina_52 Ezurina_53 Ezurina_54 Ezurina_55 Ezurina_56 Ezurina_57 Ezurina_58 Ezurina_59 Ezurina_60 Ezurina_61 Ezurina_62 Ezurina_63 Ezurina_64 Ezurina_65 Ezurina_66 Ezurina_67 Ezurina_68 Ezurina_69 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |