Mycobacterium phage FieldMouse
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Detailed Information for Phage FieldMouse | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Lauren Shebby |
Year Found | 2021 |
Location Found | Hamburg, PA United States |
Finding Institution | Lehigh University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 6°C |
GPS Coordinates | 40.567131 N, 75.95696 W Map |
Discovery Notes | On the day of soil collection, the high temperature was 44 degrees Fahrenheit and the low was 30 degrees Fahrenheit. Soil was relatively dry, but not dusty in texture. After being in a collection bag for a few days, a sapling sprouted. The sample was collected in soil adjacent to a field used for farming. |
Naming Notes | The phage was named "Field Mouse" because the location of the soil sample was chosen due to a field often being the location of decaying organisms, meaning there was likely an abundance of bacteria and therefore the presence of phage. The most prominent animal at this location is a field mouse, hence the name. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | There was variation in the head to tail ratio, so it is possible that some tails were broken, but the ratios measured were about 1:3 and 1.1:1. The tail is long and flexible and the capsid is an icosahedron shape. The plaques were turbid and symmetrical consistently throughout plaque assays. They varied in size, but were most consistently between less than a millimeter and 3 millimeters. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
Pitt Freezer Box# | 142 |
Pitt Freezer Box Grid# | H12 |
Available Files | |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |