Microbacterium phage Forever8
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| Detailed Information for Phage Forever8 | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Thu Bui |
| Year Found | 2023 |
| Location Found | Saint Louis, MO United States |
| Finding Institution | St. Louis Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 38.451512 N, 90.291865 W Map |
| Discovery Notes | The soil sample was collected at Cliff Cave Park, where the site features a 20m high limestone cliff to the west, a corn/soy field to the east and south, and a small forest and marsh surrounding a body of water. The area boasts abundant life, including small fish and an unknown 25-30cm long brown snake observed next to the sample site. The collected sample consists of thick, saturated mud with abundant living and dead plant matter and was obtained at 10:15 am. Regarding the procedures for the 2nd and 3rd serial dilutions, as well as the subsequent spot titer, contamination issues were encountered. The process had to be restarted twice, beginning again from the original sample for the final attempt. |
| Naming Notes | The name came from my hope that the phage data will be last forever and number 8 is my favorite number. Also, if flip 8 side way, we can have the infinite sign. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Lysate Titer: 2.3 x 10^3 pfu/ml DNA Concentration: 17.7 ng/µl |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Forever8 has no tail with an average capsid width of 64.14 nm. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
