Mycobacterium phage Gancho
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| Detailed Information for Phage Gancho | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | CaptainHook |
| Found By | Maile Anthony & Jessica Granados |
| Year Found | 2016 |
| Location Found | Louisville, CO USA |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.98 N, 105.13 W Map |
| Discovery Notes | Soil collected from potted tomato plant at 09:00. Temperature outside was approximately 23.8 degrees C. |
| Naming Notes | Originally, this phage was named TBolton, but upon seeing the electron microscopy image, the name was changed to CaptainHook because of the hooked curvature of the tail. However, the name CaptainHook was already used so the final name for this phage was changed to Gancho, as "gancho" is the Spanish word for hook. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 15, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2184 |
| Genome length (bp) | 54125 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 14 bases |
| Overhang Sequence | TAAGCCGCGCGGTA |
| GC Content | 67.5% |
| Sequencing Notes | There was a contaminating phage in the sample from Cluster A1. It got about 7% of the reads compared to about 93% of the reads for the Cluster Q phage. Thus, Gancho was assigned to be the Cluster Q phage, but it is possible it is a mixed lysate. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | Q |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Colorado Boulder |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques produced were clear and round, approximately 1-2 mm in diameter. Due to the complete clearing of bacteria in the plaques, it was predicted that CaptainHook is a lytic phage. |
| Morphotype | Siphoviridae |
| Number of Genes | 86 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH727549 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 39 |
| Pitt Freezer Box Grid# | F7 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
