| Detailed Information for Phage Gancho | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | CaptainHook |
| Found By | Maile Anthony & Jessica Granados |
| Year Found | 2016 |
| Location Found | Louisville, CO USA |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.98 N, 105.13 W Map |
| Discovery Notes | Soil collected from potted tomato plant at 09:00. Temperature outside was approximately 23.8 degrees C. |
| Naming Notes | Originally, this phage was named TBolton, but upon seeing the electron microscopy image, the name was changed to CaptainHook because of the hooked curvature of the tail. However, the name CaptainHook was already used so the final name for this phage was changed to Gancho, as "gancho" is the Spanish word for hook. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 15, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2184 |
| Genome length (bp) | 54125 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 14 bases |
| Overhang Sequence | TAAGCCGCGCGGTA |
| GC Content | 67.5% |
| Sequencing Notes | There was a contaminating phage in the sample from Cluster A1. It got about 7% of the reads compared to about 93% of the reads for the Cluster Q phage. Thus, Gancho was assigned to be the Cluster Q phage, but it is possible it is a mixed lysate. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | Q |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Colorado Boulder |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques produced were clear and round, approximately 1-2 mm in diameter. Due to the complete clearing of bacteria in the plaques, it was predicted that CaptainHook is a lytic phage. |
| Morphotype | Siphoviridae |
| Number of Genes | 86 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewGancho_1 Gancho_2 Gancho_3 Gancho_4 Gancho_5 Gancho_6 Gancho_7 Gancho_8 Gancho_9 Gancho_10 Gancho_11 Gancho_12 Gancho_13 Gancho_14 Gancho_15 Gancho_16 Gancho_17 Gancho_18 Gancho_19 Gancho_20 Gancho_21 Gancho_22 Gancho_23 Gancho_24 Gancho_25 Gancho_26 Gancho_27 Gancho_28 Gancho_29 Gancho_30 Gancho_31 Gancho_32 Gancho_33 Gancho_34 Gancho_35 Gancho_36 Gancho_37 Gancho_38 Gancho_39 Gancho_40 Gancho_41 Gancho_42 Gancho_43 Gancho_44 Gancho_45 Gancho_46 Gancho_47 Gancho_48 Gancho_49 Gancho_50 Gancho_51 Gancho_52 Gancho_53 Gancho_54 Gancho_55 Gancho_56 Gancho_57 Gancho_58 Gancho_59 Gancho_60 Gancho_61 Gancho_62 Gancho_63 Gancho_64 Gancho_65 Gancho_66 Gancho_67 Gancho_68 Gancho_69 Gancho_70 Gancho_71 Gancho_72 Gancho_73 Gancho_74 Gancho_75 Gancho_76 Gancho_77 Gancho_78 Gancho_79 Gancho_80 Gancho_81 Gancho_82 Gancho_83 Gancho_84 Gancho_85 Gancho_86 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH727549 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 39 |
| Pitt Freezer Box Grid# | F7 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
