Arthrobacter phage GrimEater
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| Detailed Information for Phage GrimEater | |
| Discovery Information | |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Found By | Renee Chowdhry, Phour Phage Phriends, Phage Place |
| Year Found | 2025 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.06532 N, 118.45183 W Map |
| Discovery Notes | urban area, next to sidewalk/footpath, in plant bed, could be affected by animals/insects, approx 2ft from an apartment complex Depth from which sample was taken (in cm): 5.08 cm Air temperature if sample collected outdoors (in degrees Celsius): 15.5 ℃ Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., A. atrocyaneus, and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation. AL Quarter and Lab section: FALL 2025, Section 1A Instructor: Dr. Tejas Bouklas Teaching Assistant: Gemma Stark and Sasha Xu Undergraduate Assistant(s): Chantal De La Cruz and Sabrina Team #1 Name: Phour Phage Phriends Team #1 Members: Ashley Lopez-Mejia, Callie Phung, Andrew Lau, Sophia Gnuse Team #2 Name: The Phage Place Team #2 Members: Xinghua Zhao, Judy Lee, Bakthi Karunasundera, Hinata Nayuki Team #3 Name: Phinest Phour Team #3 Members: Cian Cox, Renee Chowdhry, Gabriel Valenzuela, Jonny Zhao |
| Naming Notes | Named after a play on words for phage eating activity |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 22, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 845 |
| Genome length (bp) | 41540 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCACCGGCTT |
| GC Content | 68.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FP |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
Click to View |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | round and turbid Description of plaque morphology: mostly clear/translucent with some having turbidity, some pinpoint plaques, circular shape, halo and sharp edges Average size (and size range) diameter in mm: 1.27 mm (average) and small/medium sizes |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |