Microbacterium phage Grog
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Detailed Information for Phage Grog
Discovery Information
Isolation HostMicrobacterium paraoxydans NRRL B-24275
Found ByAyden Pyle
Year Found2023
Location FoundCullowhee, NC United States of America
Finding InstitutionWestern Carolina University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates35.310248 N, 83.187476 W Map
Discovery NotesFound in a shady spot under a bridge near a creek. The sample was from right along the water edge, so the soil was wet and somewhat gritty. It also had a lot of minerals.
Naming NotesI retrieved my soil sample from under a bridge, so I thought it would be fitting to give my phage a troll-like name.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesOriginally the plaques were very small and uniform. After I was done purifying it and was in the process of growing a stock, the morphology changed dramatically. I purified it again, but the morphology of the plaques remained the same. My instructor thinks it has something to do with her making a new batch of bacteria stock, and my phage being particularly sensitive to the change. There was another similar case in the class.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 10^10 PFU/mL
Pitt Freezer Box# 175
Pitt Freezer Box Grid# F5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload