| Detailed Information for Phage Hirko | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Hajira Choudry |
| Year Found | 2022 |
| Location Found | Lisle, IL United States |
| Finding Institution | Benedictine University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 22°C |
| GPS Coordinates | 41.775 N, 88.0985 W Map |
| Discovery Notes | The soil sample was retrieved near a tree on the outskirts of Benedictine Campus. The weather was 22.2 degrees Celsius (72 degrees Fahrenheit) and lightly cloudy with showers from the night before. Retrieving the dirt was easy since it was already a little moist; gloves were used to dig it up. The soil was fairly soft, wasn’t chunky, and didn’t have a lot of wood chips, pebbles, etc in it. I wore gloves and dug about 2 inches down to get ⅓ of a zip lock bag filled with soil. The soil looked dark brown and had roots in it since I dug next to a tree. I decided to dig here since the dirt was slightly moist and it was near a tree which is known to have a good amount of Arthrobacter phage. |
| Naming Notes | I chose this phage name because it's the first letter of my name and I wrote down whatever random word I could think of that starts with the letter "H" at the top of my head. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 21, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 659 |
| Genome length (bp) | 48792 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FL |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | Benedictine University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque has a size of 1.5 mm in diameter and 0.75 mm in radius which leans towards a larger size for a plaque. The plaque is lytic and looks like a clear circle with no fuzzy edges for the shape of it. |
| Morphotype | Myoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewHirko_1 Hirko_2 Hirko_3 Hirko_4 Hirko_5 Hirko_6 Hirko_7 Hirko_8 Hirko_9 Hirko_10 Hirko_11 Hirko_12 Hirko_13 Hirko_14 Hirko_15 Hirko_16 Hirko_17 Hirko_18 Hirko_19 Hirko_20 Hirko_21 Hirko_22 Hirko_23 Hirko_24 Hirko_25 Hirko_26 Hirko_27 Hirko_28 Hirko_29 Hirko_30 Hirko_31 Hirko_32 Hirko_33 Hirko_34 Hirko_35 Hirko_36 Hirko_37 Hirko_38 Hirko_39 Hirko_40 Hirko_41 Hirko_42 Hirko_43 Hirko_44 Hirko_45 Hirko_46 Hirko_47 Hirko_48 Hirko_49 Hirko_50 Hirko_51 Hirko_52 Hirko_53 Hirko_54 Hirko_55 Hirko_56 Hirko_57 Hirko_58 Hirko_59 Hirko_60 Hirko_61 Hirko_62 Hirko_63 Hirko_64 Hirko_65 Hirko_66 Hirko_67 Hirko_68 Hirko_69 Hirko_70 Hirko_71 Hirko_72 Hirko_73 Hirko_74 Hirko_75 Hirko_76 Hirko_77 Hirko_78 Hirko_79 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR553913 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
