Streptomyces phage Hiyaa
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Detailed Information for Phage Hiyaa
Discovery Information
Isolation HostStreptomyces lividans JI 1326
Found ByZoe Tian, Julia Lieu, Maggie Kieser
Year Found2017
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature30°C
GPS Coordinates38.6425 N, 90.3168 W Map
Discovery NotesOutside temperature: 86 degrees F
Dirt texture: dry, organic matter
Depth of sample: 2 cm
Location description: boulevard between open ends of Forest Ridge Place
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 12, 2018
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage200
Genome length (bp)83219
Character of genome endsCircularly Permuted
GC Content66.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterSingleton
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesFirst round of purification: small plaques, round, clear.

After 3 rounds of purification: small plaques (pinprick - 1mm diameter), round, cloudy.

note: plaque picture uploaded is of first round of purification
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Hiyaa_1
(helix-turn-helix DNA binding domain)

Hiyaa_2
(terminase, large subunit)

Hiyaa_3
(portal protein)

Hiyaa_4
(minor capsid protein)

Hiyaa_5

Hiyaa_6

Hiyaa_7
(major capsid protein)

Hiyaa_8

Hiyaa_9

Hiyaa_10
(methyltransferase)

Hiyaa_11

Hiyaa_12

Hiyaa_13
(minor tail protein)

Hiyaa_14
(head-to-tail adaptor)

Hiyaa_15
(head-to-tail stopper)

Hiyaa_16

Hiyaa_17

Hiyaa_18
(major tail protein)

Hiyaa_19
(tail assembly chaperone)

Hiyaa_20
(tail assembly chaperone)

Hiyaa_21
(tape measure protein)

Hiyaa_22
(minor tail protein)

Hiyaa_23

Hiyaa_24

Hiyaa_25
(minor tail protein)

Hiyaa_26

Hiyaa_27

Hiyaa_28

Hiyaa_29
(membrane protein)

Hiyaa_30
(endolysin, LysM-like)

Hiyaa_31
(membrane protein)

Hiyaa_32

Hiyaa_33

Hiyaa_34

Hiyaa_35

Hiyaa_36

Hiyaa_37
(HNH endonuclease)

Hiyaa_38

Hiyaa_39

Hiyaa_40

Hiyaa_41
(Helicase)

Hiyaa_42

Hiyaa_44

Hiyaa_45
(RecA DNA Recombinase)

Hiyaa_46

Hiyaa_47
(DNA primase)

Hiyaa_48

Hiyaa_49

Hiyaa_50
(DNA helicase)

Hiyaa_51

Hiyaa_52

Hiyaa_53

Hiyaa_54

Hiyaa_55

Hiyaa_56
(DNA polymerase I)

Hiyaa_57

Hiyaa_58

Hiyaa_59

Hiyaa_60

Hiyaa_61

Hiyaa_62

Hiyaa_63

Hiyaa_64
(peptidase)

Hiyaa_65

Hiyaa_66

Hiyaa_67
(adenylosuccinate synthetase PurA-like)

Hiyaa_68

Hiyaa_69
(deoxyuridine triphosphatase)

Hiyaa_70

Hiyaa_71
(membrane protein)

Hiyaa_72

Hiyaa_73

Hiyaa_74

Hiyaa_75

Hiyaa_76
(adenylosuccinate lyase, PurB-like)

Hiyaa_77

Hiyaa_78

Hiyaa_79

Hiyaa_80
(membrane protein)

Hiyaa_81

Hiyaa_82

Hiyaa_83

Hiyaa_84

Hiyaa_85

Hiyaa_86

Hiyaa_87
(membrane protein)

Hiyaa_88

Hiyaa_89

Hiyaa_90

Hiyaa_91

Hiyaa_92

Hiyaa_93

Hiyaa_94

Hiyaa_95
(membrane protein)

Hiyaa_96

Hiyaa_97

Hiyaa_98
(membrane protein)

Hiyaa_99

Hiyaa_100

Hiyaa_101

Hiyaa_102
(FtsK DNA translocase)

Hiyaa_103

Hiyaa_104

Hiyaa_105

Hiyaa_106

Hiyaa_107

Hiyaa_108

Hiyaa_109

Hiyaa_110

Hiyaa_111

Hiyaa_112

Hiyaa_113

Hiyaa_114

Hiyaa_115

Hiyaa_116

Hiyaa_117

Hiyaa_118

Hiyaa_119

Hiyaa_120

Hiyaa_121

Hiyaa_122

Hiyaa_123

Hiyaa_124

Hiyaa_125
(membrane protein)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK279841
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.0*10^9
Pitt Freezer Box# 49
Pitt Freezer Box Grid# C9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload