Arthrobacter phage Hum25
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Detailed Information for Phage Hum25
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByHumza Raheem
Year Found2021
Location FoundOak Brook, IL United States of America
Finding InstitutionBenedictine University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature22°C
GPS Coordinates41.824622 N, 87.988113 W Map
Discovery NotesThe sample was found from a pond close to 35th street in Oak Brook, Illinois. The skies were cloudy that day and there was no rain for the past couple of days.
The soil itself was a little wet and loose. There was some dead leaves, rocks, and twigs.
Naming NotesI used the first 3 letters of my name and the date I was born.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 19, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1287
Genome length (bp)42695
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCGGTGA
GC Content59.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterFQ
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members

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Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque NotesThe diameter with the fuzziness around it is about 4 mm. The diameter without the fuzziness is about 2 mm. In the first 24 hours after plating, the plaques do not appear that large. Around 48 hours after plating, the plaques get bigger and spread across the plate.
Number of Genes86
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Hum25_1
(terminase, small subunit)

Hum25_2
(terminase, large subunit)

Hum25_3
(portal protein)

Hum25_4

Hum25_5
(scaffolding protein)

Hum25_6
(major capsid protein)

Hum25_7

Hum25_8
(head-to-tail adaptor)

Hum25_9
(head-to-tail stopper)

Hum25_10

Hum25_11
(tail terminator)

Hum25_12

Hum25_13

Hum25_14
(tail assembly chaperone)

Hum25_15
(tail assembly chaperone)

Hum25_16

Hum25_17
(tape measure protein)

Hum25_18
(minor tail protein)

Hum25_19
(minor tail protein)

Hum25_20
(minor tail protein)

Hum25_21

Hum25_22
(minor tail protein)

Hum25_23
(endolysin)

Hum25_24
(holin)

Hum25_25

Hum25_26
(esterase)

Hum25_27
(O-acetyltransferase)

Hum25_28

Hum25_29

Hum25_30

Hum25_31

Hum25_32

Hum25_33

Hum25_34
(tyrosine integrase)

Hum25_35

Hum25_36

Hum25_37
(RNA binding protein)

Hum25_38
(helix-turn-helix DNA binding domain)

Hum25_39
(helix-turn-helix DNA binding domain)

Hum25_40

Hum25_41

Hum25_42

Hum25_43

Hum25_44
(helix-turn-helix DNA binding domain)

Hum25_45

Hum25_46

Hum25_47

Hum25_48
(ERF family DNA pairing protein)

Hum25_49
(ERF family DNA pairing protein)

Hum25_50

Hum25_51

Hum25_52

Hum25_53

Hum25_54

Hum25_55
(HNH endonuclease)

Hum25_56
(DNA methyltransferase)

Hum25_57
(RepA-like replication initiator)

Hum25_58
(DnaB-like dsDNA helicase)

Hum25_59

Hum25_60
(MazG-like nucleotide pyrophosphohydrolase)

Hum25_61
(SSB protein)

Hum25_62
(RusA-like resolvase)

Hum25_63

Hum25_64

Hum25_65

Hum25_66

Hum25_67

Hum25_68

Hum25_69

Hum25_70

Hum25_71

Hum25_72

Hum25_73

Hum25_74

Hum25_75

Hum25_76

Hum25_77
(helix-turn-helix DNA binding domain)

Hum25_78

Hum25_79

Hum25_80

Hum25_81
(protease)

Hum25_82

Hum25_83

Hum25_84

Hum25_85

Hum25_86
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPQ244012
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 6.3 x 10^10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload