| Detailed Information for Phage MrSmee | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Former names | NooNoo |
| Found By | Abigail Antonishek |
| Year Found | 2023 |
| Location Found | Schiedam, Netherlands |
| Finding Institution | Purdue University |
| Program | Unavailable |
| From enriched soil sample? | No |
| Isolation Temperature | 40°C |
| GPS Coordinates | 51.930294 N, 4.362358 E Map |
| Discovery Notes | Collection Date: 8/25/2023 Collection Time: 11pm Soil: Soil was damp (as it had rained earlier that day), full of organic material, rich in color (dark brown almost black), and made up of small particles. The sample was collected from a large flowerpot outside a hotel. Depth: 1 inch The sample was kept in a sealed ziploc bag for 4 days before direct isolation. |
| Naming Notes | MrSmee traveled the globe alongside Captain Hook. This bacteriophage was collected overseas and is no stranger to traveling. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1253 |
| Genome length (bp) | 41092 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCTCCGGCTCT |
| GC Content | 60.6% |
| Sequencing Notes | The DNA sample that was sequenced for this phage was contaminated with a small amount (~10%) of a novel Cluster FH phage. Also, it appears the overhang is 12 bases although the evidence for the right end of the genome wasn't super strong. It could be a different length potentially, but with the data we have now it looks like 12 bases. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Purdue University |
| Annotation Status | Being Annotated (Expected completion by 6/15/2024) |
| Plaque Notes | average plaque diameter of 0.2cm. Well-defined borders. Clear plaques. Perfectly circular plaques. Diameter of plaques is relatively consistent. |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewMrSmee_Draft_1 MrSmee_Draft_2 MrSmee_Draft_3 MrSmee_Draft_4 MrSmee_Draft_5 MrSmee_Draft_6 MrSmee_Draft_7 MrSmee_Draft_8 MrSmee_Draft_9 MrSmee_Draft_10 MrSmee_Draft_11 MrSmee_Draft_12 MrSmee_Draft_13 MrSmee_Draft_14 MrSmee_Draft_15 MrSmee_Draft_16 MrSmee_Draft_17 MrSmee_Draft_18 MrSmee_Draft_19 MrSmee_Draft_20 MrSmee_Draft_21 MrSmee_Draft_22 MrSmee_Draft_23 MrSmee_Draft_24 MrSmee_Draft_25 MrSmee_Draft_26 MrSmee_Draft_27 MrSmee_Draft_28 MrSmee_Draft_29 MrSmee_Draft_30 MrSmee_Draft_31 MrSmee_Draft_32 MrSmee_Draft_33 MrSmee_Draft_34 MrSmee_Draft_35 MrSmee_Draft_36 MrSmee_Draft_37 MrSmee_Draft_38 MrSmee_Draft_39 MrSmee_Draft_40 MrSmee_Draft_41 MrSmee_Draft_42 MrSmee_Draft_43 MrSmee_Draft_44 MrSmee_Draft_45 MrSmee_Draft_46 MrSmee_Draft_47 MrSmee_Draft_48 MrSmee_Draft_49 MrSmee_Draft_50 MrSmee_Draft_51 MrSmee_Draft_52 MrSmee_Draft_53 MrSmee_Draft_54 MrSmee_Draft_55 MrSmee_Draft_56 MrSmee_Draft_57 MrSmee_Draft_58 MrSmee_Draft_59 MrSmee_Draft_60 MrSmee_Draft_61 MrSmee_Draft_62 MrSmee_Draft_63 MrSmee_Draft_64 MrSmee_Draft_65 MrSmee_Draft_66 MrSmee_Draft_67 MrSmee_Draft_68 MrSmee_Draft_69 MrSmee_Draft_70 MrSmee_Draft_71 MrSmee_Draft_72 MrSmee_Draft_73 MrSmee_Draft_74 MrSmee_Draft_75 MrSmee_Draft_76 MrSmee_Draft_77 MrSmee_Draft_78 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 187 |
| Pitt Freezer Box Grid# | F10 |
| Available Files | |
| Plaque Picture | Download |
