| Detailed Information for Phage Ignacio | |
| Discovery Information | |
| Isolation Host | Streptomyces sanglieri UNT16F27A |
| Found By | Alejandro Coronado |
| Year Found | 2019 |
| Location Found | El Paso, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 31.784983 N, 106.448778 W Map |
| Discovery Notes | Collected soil from a homemade compost. The soil was covered by old grass clippings and milkweed growing around it. The temperature of the collection date was 38°C with a humidity of 20%. The soil was a mixture of sand and organic matter, a few grass clippings found within. In comparison to the rest of the soil surrounding the compost, it was darker in tone and lost the iron smell that the other soil had. |
| Naming Notes | The name was based on Saint Ignatius of Loyola. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 17, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2401 |
| Genome length (bp) | 38137 |
| Character of genome ends | Circularly Permuted |
| GC Content | 71.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BP |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | My phage had mesas and appeared to be temperate. Successfully tested but there was final mesa formed. |
| Other Cluster Members |
Click to View |
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | The phage is cloudy and round ranging in size from 1mm to smaller. |
| Number of Genes | 58 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewIgnacio_1 Ignacio_2 Ignacio_3 Ignacio_4 Ignacio_5 Ignacio_6 Ignacio_7 Ignacio_8 Ignacio_9 Ignacio_10 Ignacio_11 Ignacio_12 Ignacio_13 Ignacio_14 Ignacio_15 Ignacio_16 Ignacio_17 Ignacio_18 Ignacio_19 Ignacio_20 Ignacio_21 Ignacio_22 Ignacio_23 Ignacio_24 Ignacio_25 Ignacio_26 Ignacio_27 Ignacio_28 Ignacio_29 Ignacio_30 Ignacio_31 Ignacio_32 Ignacio_33 Ignacio_34 Ignacio_35 Ignacio_36 Ignacio_37 Ignacio_38 Ignacio_39 Ignacio_40 Ignacio_41 Ignacio_42 Ignacio_43 Ignacio_44 Ignacio_45 Ignacio_46 Ignacio_47 Ignacio_48 Ignacio_49 Ignacio_50 Ignacio_51 Ignacio_52 Ignacio_53 Ignacio_54 Ignacio_55 Ignacio_56 Ignacio_57 Ignacio_58 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT451980 |
| Refseq Number | None yet |
| Published in Paper | Nath et al 2022, Microbiology Resource Announcements: Complete Genome Sequences of the Novel Cluster BP Phages Infecting Streptomyces sanglieri, AxeJC, Cumberbatch, Eastland, Eklok, HFrancette, Ignacio, Piccadilly, and Vondra |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 4.0x10^11 pfu/ml |
| Pitt Freezer Box# | 104 |
| Pitt Freezer Box Grid# | B5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
