Microbacterium phage Johann
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| Detailed Information for Phage Johann | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Kerry Iles |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.444253 N, 79.953239 W Map |
| Naming Notes | Named after Johann Sebastian Bach |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 30, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 862 |
| Genome length (bp) | 42363 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EJ |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | The 10e-4 spot of the phage on a spot titer plate was streaked for evidence of lysogeny after four days of incubation at 37 degrees Celsius. No growth was seen on the streak, indicating this phage does not form a lysogen. |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are clear and 1-3 millimeters in diameter with turbid halos. |
| Morphotype | Siphoviridae |
| Number of Genes | 62 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK016497 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 6 |
| Pitt Freezer Box Grid# | F2 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |