| Detailed Information for Phage Johann | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Kerry Iles |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.444253 N, 79.953239 W Map |
| Naming Notes | Named after Johann Sebastian Bach |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 30, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 862 |
| Genome length (bp) | 42363 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EJ |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | The 10e-4 spot of the phage on a spot titer plate was streaked for evidence of lysogeny after four days of incubation at 37 degrees Celsius. No growth was seen on the streak, indicating this phage does not form a lysogen. |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are clear and 1-3 millimeters in diameter with turbid halos. |
| Morphotype | Siphoviridae |
| Number of Genes | 62 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewJohann_1 Johann_2 Johann_3 Johann_4 Johann_5 Johann_6 Johann_7 Johann_8 Johann_9 Johann_10 Johann_11 Johann_12 Johann_13 Johann_14 Johann_15 Johann_16 Johann_17 Johann_18 Johann_19 Johann_20 Johann_21 Johann_22 Johann_23 Johann_24 Johann_25 Johann_26 Johann_27 Johann_28 Johann_29 Johann_30 Johann_31 Johann_32 Johann_33 Johann_34 Johann_35 Johann_36 Johann_37 Johann_38 Johann_39 Johann_40 Johann_41 Johann_42 Johann_43 Johann_44 Johann_45 Johann_46 Johann_47 Johann_48 Johann_49 Johann_50 Johann_51 Johann_52 Johann_53 Johann_54 Johann_55 Johann_56 Johann_57 Johann_58 Johann_59 Johann_60 Johann_61 Johann_62 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK016497 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 6 |
| Pitt Freezer Box Grid# | F2 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |