Microbacterium phage Johann
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Detailed Information for Phage Johann
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByKerry Iles
Year Found2017
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.444253 N, 79.953239 W Map
Naming NotesNamed after Johann Sebastian Bach
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 30, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage862
Genome length (bp)42363
Character of genome endsCircularly Permuted
GC Content66.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEJ
Subcluster--
Cluster Life CycleUnknown
Lysogeny NotesThe 10e-4 spot of the phage on a spot titer plate was streaked for evidence of lysogeny after four days of incubation at 37 degrees Celsius. No growth was seen on the streak, indicating this phage does not form a lysogen.
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesPlaques are clear and 1-3 millimeters in diameter with turbid halos.
MorphotypeSiphoviridae
Number of Genes62
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK016497
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 6
Pitt Freezer Box Grid# F2
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload