Mycobacterium phage JohnnyCache
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| Detailed Information for Phage JohnnyCache | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Zach Wylie |
| Year Found | 2017 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 32°C |
| GPS Coordinates | 43.057 N, 85.454 W Map |
| Discovery Notes | The phage was discovered 8/24/17 in damp, shaded soil located in a forest. The soil was at the site of a fallen, decomposed tree and was high in organics. Humidity was 95%, and the Temperature was 13 degrees Celsius. |
| Naming Notes | This phage was discovered producing very turbid plaques among another species of phage that produced much clearer plaques. I first named these phages after the French word for hidden, "caché". I felt that this was a rather boring name, and went with Johnny Cache instead. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Johnny Cache produces small uniform plaques about 1 to 2 mm in diameter. In a serial dilution, the plaques are affected by some sort of density dependent factor. At lower dilutions, large turbid plaques are produced. At higher dilutions, smaller, more clear plaques can be found. Phage will not grow at 42 degrees Celcius. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 50 |
| Pitt Freezer Box Grid# | D6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
