Mycobacterium phage Kamu
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| Detailed Information for Phage Kamu | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Alanna Unruh and Kaylee Moniz |
| Year Found | 2016 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 47.666008 N, 117.404062 W Map |
| Discovery Notes | We discovered our phage through a soil sample taken near Lake Arthur on Gonzaga University's campus. The soil itself was somewhat wet and dark in color indicating moisture that could have been transferred over from the lake. We decided to take our sample from this spot since other groups had success with their soil samples and we figured the likelihood of finding a phage in a soil sample near a lake would be higher than elsewhere. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Our plaques were approximately 1 to 2 mm in size. They were uniform and similar in shape with being symmetric and round. They were all temperate. After receiving our TEM images, the images showed that the capsids were symmetric and round, with an approximate size of 58.33 nm or 0.7 cm. Our tail measured at 300 nm or 3.6 cm in length. The total length of our phage was 366.66 nm or 4.4 cm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 7.4x10^8 pfu/mL |
| Date of SEA Lysate Titering | Oct 13, 2016 |
| Pitt Freezer Box# | 40 |
| Pitt Freezer Box Grid# | H3 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
