Streptomyces phage Krazt
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| Detailed Information for Phage Krazt | |
| Discovery Information | |
| Isolation Host | Streptomyces sanglieri UNT16F27A |
| Found By | Richard Johnston |
| Year Found | 2020 |
| Location Found | Denton, TX America |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.21553 N, 97.132446 W Map |
| Discovery Notes | I recived a plaque from anouther student by the name of Kalonda Kapinga. After conducting Phage titer 2 I noticed 2 distict sizes in 10^-4 plate. One was around 1mm and the other was around .1mm. I decided to pick the smallest size. On Phage titer 3 I hade a webbed plate on 10^-1. This plate I flooded and used for my spot titer. Result was in 10^-6 I counted 12 individual plaques that measured around .5mm. I calculated titer at 2.4*10^9PFU/ML. Conducted MPI and the results for all three plates were over lysed. I changed the formula and reduced amount of IPL and was able to achieve results. I was absent for the results. Swapan Bhuiyan the SI of the class, flooded the plate and Mr. Mims filtered it and did a spot titer. I counted 8pfu in 10^-6. I calculated 1.6*10^9PFU/Ml |
| Naming Notes | I gave the name Krazt to my phage. It is a name that I have hade in my head since I was 13. The name was my breakdance name back when I started breakdancing when I was a kid. This was a special time in my life, so what better name to give my phage as this is also a special time as well. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 117 |
| Pitt Freezer Box Grid# | G11 |
| Available Files | |
| Plaque Picture | Download |