Mycobacterium phage Ladiana
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| Detailed Information for Phage Ladiana | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kaitlyn Lein |
| Year Found | 2017 |
| Location Found | Byron Center, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 32°C |
| GPS Coordinates | 42.7983 N, 85.6547 W Map |
| Discovery Notes | Found beneath a layer of woodchips in a garden underneath a tomato plant. It was collected on 8-24-17 at approximately 8 pm. The soil was moist. |
| Naming Notes | La diana means bullseye in Spanish, so I just combined the la and the diana to make Ladiana. This is because the morphology of my original plaques were bullseyes. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | This phage did not grow on a Xeno Lysogen plate, which reinforces the DNA analysis that this phage is likely not a cluster N phage. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Some are bullseyes with defined edges and some seem to mutate and form clear plaques with undefined edges, possibly as a result of density dependent factors or plating using a lysate versus plaque picking. Plaque morphology changes when grown at 42°C to a large, slightly turbid circle with a halo. A time of infection test resulted in a slight increase in plaques with increased time before plating, however the data was not consistent and the procedure would need to be repeated before concluding that increased time of infection leads to increase plaques. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 50 |
| Pitt Freezer Box Grid# | D7 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
