Microbacterium phage LittleHoudini
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Detailed Information for Phage LittleHoudini
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByGeorgia Matheson
Year Found2018
Location FoundCullowhee, NC USA
Finding InstitutionWestern Carolina University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates35.302778 N, 83.183611 W Map
Discovery NotesSample taken from the surface of the soil just underneath running water in Cullowhee Creek.
Naming NotesThe name "LittleHoudini" came from the disappearing act that the phage performed during discovery. When growing a new batch of bacteria to use in our discovery/characterization experiments, the phage revealed a great sensitivity to a change in the type of peptone and disappeared for a while until the bacteria was grown the original way(or at least close to it).
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe morphology of LittleHoudini at the beginning was small and very clear, but after messing with the formula of the bacterial growth agents, the plaques remained a familiar size, but took on a bit of a hazy glaze instead of being perfectly transparent as they were.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1x10^9 PFU/mL
Pitt Freezer Box# 81
Pitt Freezer Box Grid# C10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload