| Detailed Information for Phage Luna22 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Megan Edwards |
| Year Found | 2018 |
| Location Found | Charleston, SC USA |
| Finding Institution | College of Charleston |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.784094 N, 79.935882 W Map |
| Discovery Notes | Found under a tree in dark soil. |
| Naming Notes | The name Luna is from Megan Edwards' coach. Her coach’s last name is Moon. And 22 has always been Megan’s jersey number in sports. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 5, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2352 |
| Genome length (bp) | 53730 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 14 bases |
| Overhang Sequence | TAAGCCGCGCGGTA |
| GC Content | 67.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | Q |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | College of Charleston |
| Annotation Status | In GenBank |
| Plaque Notes | Phage came from a small, round, and clear plaque about 2-3mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 78 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW924639 |
| Refseq Number | None yet |
| Published in Paper | Flota et al 2021, Microbiology Resource Announcements: Evaluation of Genome Sequences of the Bacteriophages JeTaime and Luna22 |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
93 |
| Pitt Freezer Box Grid# |
E4 |
| Available Files |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |