Mycobacterium phage Marisole
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| Detailed Information for Phage Marisole | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ariel Lowe |
| Year Found | 2021 |
| Location Found | Caneyville, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.335699 N, 86.508758 W Map |
| Discovery Notes | An enrichment was created by introducing the soil sample to the host, Mycobacterium smegmatis. The enrichment was then used as the starting sample for the serial dilutions. Five serial dilutions were conducted to purify the phage. |
| Naming Notes | I decided upon the name Marisole from the film, The Cheetah Girls 2. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | A |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | My plaques were roughly 3.3mm. My plaques were also turbid and circular. During the first half of my experiment, plaques were being displayed across the plates. Over time, plaques were not being seen. After multiple rounds of serial dilutions, it was determined that my plates displayed clear-forming plaques. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5.5 x 10^6 pfu/mL |
| Pitt Freezer Box# | 137 |
| Pitt Freezer Box Grid# | H4 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
