Arthrobacter phage MooKitty
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| Detailed Information for Phage MooKitty | |
| Discovery Information | |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Found By | Zoe Gleason |
| Year Found | 2022 |
| Location Found | Los Angeles, CA US |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.2811 N, 118.4401 W Map |
| Discovery Notes | Isolated by TAs Dominic Garza and Michelle Zorawik An enriched culture was prepared by combining 15 mL of soil with 25 mL of PYCA broth. The bacterial strains of A. sp., A. globiformis, A. sulfureus and A. atrocyaneus were then added in a ratio of 1:5:192:588 respectively. The enriched culture was incubated at 30℃ for 48 hours with shaking. After filtering with a 0.22 µm filter, the sample was plated on PYCA plates with 2X PYCa soft agar using the standard pour plate method. |
| Naming Notes | This phage was named in honor of the discoverer’s cat, Moo. She has many different nicknames, but MooKitty is the most used. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 8, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 5585 |
| Genome length (bp) | 15972 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCCGCGCCCC |
| GC Content | 53.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AX |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Halo with a clear center and turbid edges. 12 mm diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewMooKitty_1 MooKitty_2 MooKitty_3 MooKitty_4 MooKitty_5 MooKitty_6 MooKitty_7 MooKitty_8 MooKitty_9 MooKitty_10 MooKitty_11 MooKitty_12 MooKitty_13 MooKitty_14 MooKitty_15 MooKitty_16 MooKitty_17 MooKitty_18 MooKitty_19 MooKitty_20 MooKitty_21 MooKitty_22 MooKitty_23 MooKitty_24 MooKitty_25 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR613479 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 155 |
| Pitt Freezer Box Grid# | H7 |
| Available Files | |
| GenBank File for Phamerator | Download |