Arthrobacter phage MooKitty
MooKitty Plaque Picture
MooKitty EM Picture
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Detailed Information for Phage MooKitty
Discovery Information
Isolation HostArthrobacter sulfureus ATCC 19098
Found ByZoe Gleason
Year Found2022
Location FoundLos Angeles, CA US
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature30°C
GPS Coordinates34.2811 N, 118.4401 W Map
Discovery NotesIsolated by TAs Dominic Garza and Michelle Zorawik

An enriched culture was prepared by combining 15 mL of soil with 25 mL of PYCA broth. The bacterial strains of A. sp., A. globiformis, A. sulfureus and A. atrocyaneus were then added in a ratio of 1:5:192:588 respectively. The enriched culture was incubated at 30℃ for 48 hours with shaking. After filtering with a 0.22 µm filter, the sample was plated on PYCA plates with 2X PYCa soft agar using the standard pour plate method.
Naming NotesThis phage was named in honor of the discoverer’s cat, Moo. She has many different nicknames, but MooKitty is the most used.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 8, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage5585
Genome length (bp)15972
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCCCGCGCCCC
GC Content53.8%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members

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Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque NotesHalo with a clear center and turbid edges. 12 mm diameter.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR613479
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 155
Pitt Freezer Box Grid# H8
Available Files
GenBank File for PhameratorDownload