Arthrobacter phage MrSmee
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| Detailed Information for Phage MrSmee | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Former names | NooNoo |
| Found By | Abigail Antonishek |
| Year Found | 2023 |
| Location Found | Schiedam, Netherlands |
| Finding Institution | Purdue University |
| Program | Unavailable |
| From enriched soil sample? | No |
| Isolation Temperature | 40°C |
| GPS Coordinates | 51.930294 N, 4.362358 E Map |
| Discovery Notes | Collection Date: 8/25/2023 Collection Time: 11pm Soil: Soil was damp (as it had rained earlier that day), full of organic material, rich in color (dark brown almost black), and made up of small particles. The sample was collected from a large flowerpot outside a hotel. Depth: 1 inch The sample was kept in a sealed ziploc bag for 4 days before direct isolation. |
| Naming Notes | MrSmee traveled the globe alongside Captain Hook. This bacteriophage was collected overseas and is no stranger to traveling. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1253 |
| Genome length (bp) | 41092 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCTCCGGCTCT |
| GC Content | 60.6% |
| Sequencing Notes | The DNA sample that was sequenced for this phage was contaminated with a small amount (~10%) of a novel Cluster FH phage. Also, it appears the overhang is 12 bases although the evidence for the right end of the genome wasn't super strong. It could be a different length potentially, but with the data we have now it looks like 12 bases. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Purdue University |
| Annotation Status | Awaiting_QC |
| Plaque Notes | average plaque diameter of 0.2cm. Well-defined borders. Clear plaques. Perfectly circular plaques. Diameter of plaques is relatively consistent. |
| Number of Genes | 78 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewMrSmee_1 MrSmee_2 MrSmee_3 MrSmee_4 MrSmee_5 MrSmee_6 MrSmee_7 MrSmee_8 MrSmee_9 MrSmee_10 MrSmee_11 MrSmee_12 MrSmee_13 MrSmee_14 MrSmee_15 MrSmee_16 MrSmee_17 MrSmee_18 MrSmee_19 MrSmee_20 MrSmee_21 MrSmee_22 MrSmee_23 MrSmee_24 MrSmee_25 MrSmee_26 MrSmee_27 MrSmee_28 MrSmee_29 MrSmee_30 MrSmee_31 MrSmee_32 MrSmee_33 MrSmee_34 MrSmee_35 MrSmee_36 MrSmee_37 MrSmee_38 MrSmee_39 MrSmee_40 MrSmee_41 MrSmee_42 MrSmee_43 MrSmee_44 MrSmee_45 MrSmee_46 MrSmee_47 MrSmee_48 MrSmee_49 MrSmee_50 MrSmee_51 MrSmee_52 MrSmee_53 MrSmee_54 MrSmee_55 MrSmee_56 MrSmee_57 MrSmee_58 MrSmee_59 MrSmee_60 MrSmee_61 MrSmee_62 MrSmee_63 MrSmee_64 MrSmee_65 MrSmee_66 MrSmee_67 MrSmee_68 MrSmee_69 MrSmee_70 MrSmee_71 MrSmee_72 MrSmee_73 MrSmee_74 MrSmee_75 MrSmee_76 MrSmee_77 MrSmee_78 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 187 |
| Pitt Freezer Box Grid# | F10 |
| Available Files | |
| Plaque Picture | Download |
