Mycobacterium phage Muck
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Detailed Information for Phage Muck
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEli Mucker
Year Found2023
Location FoundOwensboro, KY United States of America
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates37.743944 N, 87.056778 W Map
Discovery NotesThe bacteriophage was discovered in an arid, light brown soil environment heavily scattered with debris and leaf litter, adjacent to residential areas. The soil, noticeably desiccated and exhibiting a cracked, flaky texture, suggested a prolonged absence of moisture, possibly enduring several weeks without significant hydration. I collected my sample when it was 97-degrees Fahrenheit, the location was shaded. To collect the sample, only the top layer, approximately 2-3cm deep, was gathered. This selective sampling aimed to target the surface microbial community, thriving in the challenging and water-deprived conditions prevalent in this habitat.
Naming NotesThe name "Muck" for this bacteriophage perfectly encapsulates its roots in dirt and rubbish, echoing its origin. It's a fitting name not just because of the environment it was found in but also because it's a nod to my last name, Mucker. I felt a personal connection to this discovery, so naming it after me in a way feels like leaving a mark on the scientific journey.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe plaques formed by this phage display a turbid appearance with barely noticeable, organic circular more turbid halos. On average, these plaques measure around 4-5 millimeters in diameter. After a 48-hour incubation period at 30 degrees Celsius, these characteristics become evident allowing easy observation.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 10^-8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload