Mycobacterium phage Muck
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|Detailed Information for Phage Muck
|Mycobacterium smegmatis mc²155
|Owensboro, KY United States of America
|Western Kentucky University
|Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
|From enriched soil sample?
|37.743944 N, 87.056778 W Map
|The bacteriophage was discovered in an arid, light brown soil environment heavily scattered with debris and leaf litter, adjacent to residential areas. The soil, noticeably desiccated and exhibiting a cracked, flaky texture, suggested a prolonged absence of moisture, possibly enduring several weeks without significant hydration. I collected my sample when it was 97-degrees Fahrenheit, the location was shaded. To collect the sample, only the top layer, approximately 2-3cm deep, was gathered. This selective sampling aimed to target the surface microbial community, thriving in the challenging and water-deprived conditions prevalent in this habitat.
|The name "Muck" for this bacteriophage perfectly encapsulates its roots in dirt and rubbish, echoing its origin. It's a fitting name not just because of the environment it was found in but also because it's a nod to my last name, Mucker. I felt a personal connection to this discovery, so naming it after me in a way feels like leaving a mark on the scientific journey.
|Genome length (bp)
|Character of genome ends
|Fasta file available?
|Unknown or unassigned
|The plaques formed by this phage display a turbid appearance with barely noticeable, organic circular more turbid halos. On average, these plaques measure around 4-5 millimeters in diameter. After a 48-hour incubation period at 30 degrees Celsius, these characteristics become evident allowing easy observation.
|Has been Phamerated?
|Uploaded to GenBank?
|Not in Pitt Archives
|SEA Lysate Titer
|Restriction Digest Picture