| Detailed Information for Phage Muddy | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Lilli Holst |
| Year Found | 2010 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.8587 S, 31.0218 E Map |
| Discovery Notes | Muddy was the 1400th phage entered into the Mycobacteriophage DataBase! This sample was scraped from the underside of a partially decomposed aubergine and was therefore largely decomposed vegetable matter. The sample was dark, moist, and worms and other insects were present. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 27, 2012 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent Sequencing |
| Approximate Shotgun Coverage | 400 |
| Sanger Finishing Reactions | 8 |
| Genome length (bp) | 48229 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGTAGCGGCTT |
| GC Content | 58.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Pinprick sized clear circular plaques, but some lessening of turbidity in one direction in some cases. |
| Morphotype | Siphoviridae |
| Number of Genes | 71 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewMuddy_1 Muddy_2 Muddy_3 Muddy_4 Muddy_5 Muddy_6 Muddy_7 Muddy_8 Muddy_9 Muddy_10 Muddy_11 Muddy_12 Muddy_13 Muddy_14 Muddy_15 Muddy_16 Muddy_18 Muddy_17 Muddy_19 Muddy_20 Muddy_21 Muddy_22 Muddy_23 Muddy_24 Muddy_25 Muddy_26 Muddy_27 Muddy_28 Muddy_29 Muddy_30 Muddy_31 Muddy_32 Muddy_33 Muddy_34 Muddy_35 Muddy_36 Muddy_37 Muddy_38 Muddy_39 Muddy_40 Muddy_41 Muddy_42 Muddy_43 Muddy_44 Muddy_45 Muddy_46 Muddy_47 Muddy_48 Muddy_49 Muddy_50 Muddy_51 Muddy_52 Muddy_53 Muddy_54 Muddy_55 Muddy_56 Muddy_57 Muddy_58 Muddy_59 Muddy_60 Muddy_61 Muddy_62 Muddy_63 Muddy_64 Muddy_65 Muddy_66 Muddy_67 Muddy_68 Muddy_69 Muddy_70 Muddy_71 |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KF024728 |
| Refseq Number | NC_022054 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 2 |
| Pitt Freezer Box Grid# | D11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |