| Detailed Information for Phage Nergal | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jacob Harris |
| Year Found | 2016 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.93262 N, 86.40674 W Map |
| Discovery Notes | This soil sample was collected by Dr. Rodney King from his compost pile and was rich in decaying matter. |
| Naming Notes | Named after the Babylonian god of war and pestilence; a reference to the metaphorical war between bacteria and bacteriophages. In addition, Nergal was named after a Babylonian God in respect to the advances the Babylonians made in Math and Science. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 6, 2023 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 269 |
| Genome length (bp) | 55792 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCAAGGGGCCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AG |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
Click to View |
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are clear, reasonably well spaced, 7.5 mm in diameter, and tend to grow in lines. |
| Number of Genes | 84 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewNergal_1 Nergal_2 Nergal_3 Nergal_4 Nergal_5 Nergal_6 Nergal_7 Nergal_8 Nergal_9 Nergal_10 Nergal_11 Nergal_12 Nergal_13 Nergal_14 Nergal_15 Nergal_16 Nergal_17 Nergal_18 Nergal_19 Nergal_20 Nergal_21 Nergal_22 Nergal_23 Nergal_24 Nergal_25 Nergal_26 Nergal_27 Nergal_28 Nergal_29 Nergal_30 Nergal_31 Nergal_32 Nergal_33 Nergal_34 Nergal_35 Nergal_36 Nergal_37 Nergal_38 Nergal_39 Nergal_40 Nergal_41 Nergal_42 Nergal_43 Nergal_44 Nergal_45 Nergal_46 Nergal_47 Nergal_48 Nergal_49 Nergal_50 Nergal_51 Nergal_52 Nergal_53 Nergal_54 Nergal_55 Nergal_56 Nergal_57 Nergal_58 Nergal_59 Nergal_60 Nergal_61 Nergal_62 Nergal_63 Nergal_64 Nergal_65 Nergal_66 Nergal_67 Nergal_68 Nergal_69 Nergal_70 Nergal_71 Nergal_72 Nergal_73 Nergal_74 Nergal_75 Nergal_76 Nergal_77 Nergal_78 Nergal_79 Nergal_80 Nergal_81 Nergal_82 Nergal_83 Nergal_84 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR253904 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2016WEKUnergalJH |
| SEA Lysate Titer | 6.9 x 10^8 pfu/mL |
| Pitt Freezer Box# | 37 |
| Pitt Freezer Box Grid# | C12 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
