| Detailed Information for Phage Nergal |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jacob Harris |
| Year Found | 2016 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.93262 N, 86.40674 W Map |
| Discovery Notes | This soil sample was collected by Dr. Rodney King from his compost pile and was rich in decaying matter. |
| Naming Notes | Named after the Babylonian god of war and pestilence; a reference to the metaphorical war between bacteria and bacteriophages. In addition, Nergal was named after a Babylonian God in respect to the advances the Babylonians made in Math and Science. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 6, 2023 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 269 |
| Genome length (bp) | 55792 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCAAGGGGCCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AG |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are clear, reasonably well spaced, 7.5 mm in diameter, and tend to grow in lines. |
| Number of Genes | 84 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR253904 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2016WEKUnergalJH |
| SEA Lysate Titer |
6.9 x 10^8 pfu/mL |
| Pitt Freezer Box# |
37 |
| Pitt Freezer Box Grid# |
C12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
