Mycobacterium phage NoShow
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Detailed Information for Phage NoShow | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Danielle Niblock |
Year Found | 2021 |
Location Found | Philadelphia, PA United States |
Finding Institution | Saint Joseph's University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Unknown |
Isolation Temperature | 37°C |
GPS Coordinates | 39.996504 N, 75.245769 W Map |
Discovery Notes | The soil sample was found at Saint Joseph's University on the Merion side of campus. The date was January 25th 2021 and the time of the collection was 12:45 pm. It was to the far right of the baseball field on the slope of a hill under some trees. It was -1.1 degrees Celsius outside where the soil sample was collected. The weather condition was sunny, windy, and dry. The humidity was 40%. The soil condition was hard and wet. |
Naming Notes | The phage is named NoShow because it took many trials of purification round 1 to get plaques on the plate. After purification round 1 it took the purification of many different plaques until plaques continued to show up on purification rounds 2 and 3. |
Sequencing Information | |
Sequencing Complete? | Yes |
Date Sequencing Completed | Aug 9, 2021 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 154 |
Genome length (bp) | 52825 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | CGGAGAGGCTT |
GC Content | 60.5% |
Fasta file available? | Yes: Download fasta file |
Characterization | |
Cluster | AB |
Subcluster | -- |
Cluster Life Cycle | Lytic |
Other Cluster Members | |
Annotating Institution | Saint Joseph's University |
Annotation Status | In GenBank |
Number of Genes | 80 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List | |
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info | |
Uploaded to GenBank? | Yes |
GenBank Accession | ON108645 |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
Pitt Freezer Box# | 129 |
Pitt Freezer Box Grid# | H11 |
Available Files | |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |