| Detailed Information for Phage NoShow | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Danielle Niblock |
| Year Found | 2021 |
| Location Found | Philadelphia, PA United States |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 37°C |
| GPS Coordinates | 39.996504 N, 75.245769 W Map |
| Discovery Notes | The soil sample was found at Saint Joseph's University on the Merion side of campus. The date was January 25th 2021 and the time of the collection was 12:45 pm. It was to the far right of the baseball field on the slope of a hill under some trees. It was -1.1 degrees Celsius outside where the soil sample was collected. The weather condition was sunny, windy, and dry. The humidity was 40%. The soil condition was hard and wet. |
| Naming Notes | The phage is named NoShow because it took many trials of purification round 1 to get plaques on the plate. After purification round 1 it took the purification of many different plaques until plaques continued to show up on purification rounds 2 and 3. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 9, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 154 |
| Genome length (bp) | 52825 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGAGAGGCTT |
| GC Content | 60.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Saint Joseph's University |
| Annotation Status | In GenBank |
| Number of Genes | 80 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewNoShow_1 NoShow_2 NoShow_3 NoShow_4 NoShow_5 NoShow_6 NoShow_7 NoShow_8 NoShow_9 NoShow_10 NoShow_11 NoShow_12 NoShow_13 NoShow_14 NoShow_15 NoShow_16 NoShow_17 NoShow_18 NoShow_19 NoShow_20 NoShow_21 NoShow_22 NoShow_23 NoShow_24 NoShow_25 NoShow_26 NoShow_27 NoShow_28 NoShow_29 NoShow_30 NoShow_31 NoShow_32 NoShow_33 NoShow_34 NoShow_35 NoShow_36 NoShow_37 NoShow_38 NoShow_39 NoShow_40 NoShow_41 NoShow_42 NoShow_43 NoShow_44 NoShow_45 NoShow_46 NoShow_47 NoShow_48 NoShow_49 NoShow_50 NoShow_51 NoShow_52 NoShow_53 NoShow_54 NoShow_55 NoShow_56 NoShow_57 NoShow_58 NoShow_59 NoShow_60 NoShow_61 NoShow_62 NoShow_63 NoShow_64 NoShow_65 NoShow_66 NoShow_67 NoShow_68 NoShow_69 NoShow_70 NoShow_71 NoShow_72 NoShow_73 NoShow_74 NoShow_75 NoShow_76 NoShow_77 NoShow_78 NoShow_79 NoShow_80 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON108645 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 129 |
| Pitt Freezer Box Grid# | H11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
