Streptomyces phage Pecan
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| Detailed Information for Phage Pecan | |
| Discovery Information | |
| Isolation Host | Streptomyces avermitilis NRRL 8165 |
| Found By | Sara Ladha |
| Year Found | 2023 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 30°C |
| GPS Coordinates | 45.401728 N, 75.652777 W Map |
| Discovery Notes | Collected from soil near tree roots in a picnic area. An ONT MinION, R10.4.1 chemistry was used to sequence genomic DNA. The obtained sequence was comprised of 10 contigs ranging from 2,228bp to 3,603bp in length, for a total of 25,634bp. 9 contigs (2248bp, 2266bp, 2291bp, 2408bp, 2530bp, 2552bp, 2651bp, 2857bp, and 3603bp) matched the Streptomyces phage Provolone (BD3) with an average of 88.2% query cover and 86.2% identity over the 9 contigs. 1 contig (2228bp) matched the Arthrobacter phage Emotion (AZ4) with 99% query and 90.91% identity, and we hypothesize this contig arose from contamination or misreads of a phage sequenced in the same flow cell. |
| Naming Notes | Found under a tree with pecan-coloured leaves. Also, this phage was named around Thanksgiving when pecan pie is commonly served. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Clear, circular, small-to-medium sized plaques with slightly bumpy borders. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 176 |
| Pitt Freezer Box Grid# | F6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
