| Detailed Information for Phage Typhonomachy |
| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Naomi Whiteside & Matthew Jacoby |
| Year Found | 2023 |
| Location Found | Fairfax, VA The United States of America |
| Finding Institution | George Mason University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 23°C |
| GPS Coordinates | 38.82968 N, 77.305838 W Map |
| Discovery Notes | Naomi isolated the phage from mulchy soil after a short rain period. The soil sample was collected approximately 4 meters from Planetary hall and 6 meters from the entrance to Exploratory hall. |
| Naming Notes | The Typhon is a mythological creature in Greek mythology. It is known for being the last and greatest battle of Zeus in his claim to the ruler of the Gods. We spent 4 weeks trying to isolate this phage and have met struggles of contamination of the bacteria and media as well as poor amplification of the phage. Given this is our Senior year, we found this a fitting name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 16, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1949 |
| Genome length (bp) | 46294 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCTT |
| GC Content | 60.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CT |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | George Mason University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2024) |
| Plaque Notes | Plaques are about 1 mm in diameter and are turbid suggesting a temperate phage. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.38 E09 |
| Pitt Freezer Box# |
170 |
| Pitt Freezer Box Grid# |
A1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
