Gordonia phage Typhonomachy
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Detailed Information for Phage Typhonomachy
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Found ByNaomi Whiteside & Matthew Jacoby
Year Found2023
Location FoundFairfax, VA The United States of America
Finding InstitutionGeorge Mason University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature23°C
GPS Coordinates38.82968 N, 77.305838 W Map
Discovery NotesNaomi isolated the phage from mulchy soil after a short rain period. The soil sample was collected approximately 4 meters from Planetary hall and 6 meters from the entrance to Exploratory hall.
Naming NotesThe Typhon is a mythological creature in Greek mythology. It is known for being the last and greatest battle of Zeus in his claim to the ruler of the Gods. We spent 4 weeks trying to isolate this phage and have met struggles of contamination of the bacteria and media as well as poor amplification of the phage. Given this is our Senior year, we found this a fitting name.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 16, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1949
Genome length (bp)46294
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTAGGCTT
GC Content60.1%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionGeorge Mason University
Annotation StatusOverdue (Expected completion by 5/1/2024)
Plaque NotesPlaques are about 1 mm in diameter and are turbid suggesting a temperate phage.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.38 E09
Pitt Freezer Box# 170
Pitt Freezer Box Grid# A1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload