| Detailed Information for Phage Phishy | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Jewel Hicks |
| Year Found | 2019 |
| Location Found | West Chester, OH Butler |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.339349 N, 84.383222 W Map |
| Discovery Notes | The soil sample was collected from a potted succulent on September 2, 2019. The plant had been exposed to rain the previous night, so the soil was slightly wet. The soil was originally collected for the plant midday on August 16, 2019 from a garden in West Chester, Ohio. The weather that day was warm and sunny and the soil was dry. There was not vegetation near the place where the soil was originally collected at the time of collection. |
| Naming Notes | The name "Phishy" comes from the word "fishy" but with a "ph" in place of the f because of the "ph" in phage. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 24, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1563 |
| Genome length (bp) | 43061 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCGGGGGA |
| GC Content | 67.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DT |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | University of Ibadan |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques for the phage are small, round, and slightly turbid. |
| Morphotype | Siphoviridae |
| Number of Genes | 58 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPhishy_1 Phishy_2 Phishy_3 Phishy_4 Phishy_5 Phishy_6 Phishy_7 Phishy_8 Phishy_9 Phishy_10 Phishy_11 Phishy_12 Phishy_13 Phishy_14 Phishy_15 Phishy_16 Phishy_17 Phishy_18 Phishy_19 Phishy_20 Phishy_21 Phishy_22 Phishy_23 Phishy_24 Phishy_25 Phishy_26 Phishy_27 Phishy_28 Phishy_29 Phishy_30 Phishy_31 Phishy_32 Phishy_33 Phishy_34 Phishy_35 Phishy_36 Phishy_37 Phishy_38 Phishy_39 Phishy_40 Phishy_41 Phishy_42 Phishy_43 Phishy_44 Phishy_45 Phishy_46 Phishy_47 Phishy_48 Phishy_49 Phishy_50 Phishy_51 Phishy_52 Phishy_53 Phishy_54 Phishy_55 Phishy_56 Phishy_57 Phishy_58 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW862986 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 3 x 10^8 |
| Pitt Freezer Box# | 115 |
| Pitt Freezer Box Grid# | A1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
