| Detailed Information for Phage PineapplePizza | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Juan Brito |
| Year Found | 2020 |
| Location Found | Amherst, MA United States |
| Finding Institution | University of Massachusetts Amherst |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | -3°C |
| GPS Coordinates | 42.3747 N, 72.5196 W Map |
| Discovery Notes | In front of Bank of America near a bus stop, collected from surface |
| Naming Notes | My favorite pizza topping |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 9214 |
| Genome length (bp) | 16662 |
| Character of genome ends | Covalent Terminal Protein |
| End Determination Notes | Though not yet experimentally verified, we believe that it's likely that this phage's genome ends are covalently bound to a Terminal Protein when in the capsid. The evidence for this is the similarity to other phages that have similar ends. Also, no sequencing reads continued past the genome ends, which is unusual in the case of a genome with 3' or 5' genome extensions, and there is a 101-bp inverted repeat at the ends of the genome. |
| GC Content | 53.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
Click to ViewArV2 Arzan Attoomi Cantare ChewyVIII Chymera Curie DocB7 DS6A E3 EmiRose Eyre Finch Finkle Footloose FuzzBuster GAL1 Gilgamesh GMA1 GMA4 Gudmit Hum25 Ibantik IdentityCrisis Jace Juicebox Kromp Kumao LilSpotty LuckyBarnes Magritte MalagasyRose Min1 MooMoo mu16 OnionKnight OscarSo phiAsp2 phiSAV Pine5 PineapplePizza PSonyx Pumpernickel pZL12 REQ2 REQ3 Reynauld RRH1 Shambre1 Shocker Sleepyhead Sparky Spartoi Success TJE1 TPA2 TPA4 TripleJ Trogglehumper TurkishDelight ValentiniPuff VanLee Whack Wollypog |
| Annotating Institution | University of Massachusetts Amherst |
| Annotation Status | In GenBank |
| Morphotype | Podoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPineapplePizza_1 PineapplePizza_2 PineapplePizza_3 PineapplePizza_4 PineapplePizza_5 PineapplePizza_6 PineapplePizza_7 PineapplePizza_8 PineapplePizza_9 PineapplePizza_10 PineapplePizza_11 PineapplePizza_12 PineapplePizza_13 PineapplePizza_14 PineapplePizza_15 PineapplePizza_16 PineapplePizza_17 PineapplePizza_18 PineapplePizza_19 PineapplePizza_20 PineapplePizza_21 PineapplePizza_22 PineapplePizza_23 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON724010 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 124 |
| Pitt Freezer Box Grid# | B4 |
| Available Files | |
| GenBank File for Phamerator | Download |