Arthrobacter phage Pitbull
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Detailed Information for Phage Pitbull
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByMichael Perrin and Thanh Nguyen
Year Found2023
Location FoundWest Lafayette, IN United States of America
Finding InstitutionPurdue University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature28°C
GPS Coordinates40.42197 N, 86.9163 W Map
Discovery NotesThe sample was collected on September 5, 2023. This sample was obtained at 3:20 pm Eastern Daylight Time from soil under a tree on the southeast corner of the Biochemistry building at Purdue University. The weather was mostly cloudy with a temperature of 88 degrees Fahrenheit (33 degrees Celsius) and a humidity of 62% The sample was approximately 4 cm deep in the soil.
Naming NotesThe phage has been named "Pitbull" after the influential music artist. This phage was gathered from a soil sample, and as Pitbull once said, "every day above ground is a great day, remember that," thus meaning that, for this phage, today is, in fact, a great day.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 3, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage2420
Genome length (bp)41461
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGCAGGGTGT
GC Content59.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterFQ
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members

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Annotating InstitutionPurdue University
Annotation StatusIn GenBank
Plaque NotesThe plaques were circular with smooth edges. The plaques resembled the bull's eye shape, and they were about 5 mm in diameter.
Number of Genes79
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Pitbull_1

Pitbull_2
(terminase)

Pitbull_3
(portal protein)

Pitbull_4
(capsid maturation protease)

Pitbull_5
(scaffolding protein)

Pitbull_6
(major capsid protein)

Pitbull_7

Pitbull_8

Pitbull_9
(head-to-tail stopper)

Pitbull_10

Pitbull_11
(tail terminator)

Pitbull_12

Pitbull_13
(major tail protein)

Pitbull_14
(tail assembly chaperone)

Pitbull_15
(tail assembly chaperone)

Pitbull_16
(tape measure protein)

Pitbull_17
(minor tail protein)

Pitbull_18
(minor tail protein)

Pitbull_19
(minor tail protein)

Pitbull_20

Pitbull_21
(minor tail protein)

Pitbull_22
(Endolysin)

Pitbull_23
(Holin)

Pitbull_24

Pitbull_25
(acetyltransferase)

Pitbull_26

Pitbull_27
(HNH endonuclease)

Pitbull_28

Pitbull_29

Pitbull_30

Pitbull_31

Pitbull_32

Pitbull_33
(Tyrosine Integrase)

Pitbull_34

Pitbull_35

Pitbull_36

Pitbull_37
(helix-turn-helix DNA binding domain)

Pitbull_38
(helix-turn-helix DNA binding domain)

Pitbull_39

Pitbull_40

Pitbull_41

Pitbull_42
(excise)

Pitbull_43

Pitbull_44

Pitbull_45

Pitbull_46

Pitbull_47
(DNA binding protein)

Pitbull_48

Pitbull_49

Pitbull_50

Pitbull_51

Pitbull_52

Pitbull_53
(HNH endonuclease)

Pitbull_54
(DNA methyltransferase)

Pitbull_55
(RepA-like replication initiator)

Pitbull_56
(DnaB-like dsDNA helicase)

Pitbull_57

Pitbull_58
(thioredoxin)

Pitbull_59
(MazG-like nucleotide pyrophosphohydrolase)

Pitbull_60
(SSB protein)

Pitbull_61

Pitbull_62

Pitbull_63

Pitbull_64

Pitbull_65

Pitbull_66

Pitbull_67

Pitbull_68

Pitbull_69

Pitbull_70

Pitbull_71

Pitbull_72

Pitbull_73

Pitbull_74
(helix-turn-helix DNA binding domain)

Pitbull_75

Pitbull_76

Pitbull_77
(Protease)

Pitbull_78

Pitbull_79
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPQ184828
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 187
Pitt Freezer Box Grid# G3
Available Files
GenBank File for PhameratorDownload