Mycobacterium phage Pollock
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| Detailed Information for Phage Pollock | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Reese Shufelt |
| Year Found | 2025 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 45.32305 N, 75.89404 W Map |
| Discovery Notes | This phage was found from soil under my friend's grandparent's compost bin, ambient temperature 20ºC. Genomic DNA was sequenced on a ONT Minion, R10.4.1 chemistry using rapid barcoding V14 and PCR amplification. The sequence was assembled into one contig of 17,594 bp with 100% query cover, 99.6% identity to Jeon (W). This sequence appears to be incomplete based on its shorter length than to cluster W phages showing close alignment. |
| Naming Notes | I named my phage Pollock after Jackson Pollock's 1950 painting: 'No.1 Lavender Mist'. I like this painting, and the splatters reminded me of my splatter-looking plaques. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Phage appears fully lytic. Transparent, non-turbid plaques with well defined margins. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaque size ranges from 0.5-1.0mm in diameter, with clear defined margins. Fully lysed plaques are shown with relatively uniform circular shape. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 7.0*10^10pfu/mL |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |