Corynebacterium phage Poushou
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Detailed Information for Phage Poushou
Discovery Information
Isolation HostCorynebacterium vitaeruminis CAG1
Duplicates of this PhageKayton
Found ByCarlos Guerrero
Year Found2011
Location Found USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.44417 N, 79.95306 W Map
Discovery NotesProphage of Corynebacterium vitaeruminis NCIB 9291.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 15, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage310
Sanger Finishing Reactions14
Genome length (bp)40353
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCACCCAT
End Determination Notes(ORIGINAL NOTES: No buildups of reads seen from shotgun sequencing.)

From the initial Ion Torrent sequencing, there were no obvious coverage dips/increases and/or buildups of read starts/ends, and so this phage's genome was assumed to be circularly permuted. Later Illumina sequencing of other phages in this cluster has shown clearly defined genome ends with a 10-base 3' overhang. The correction to this phage's information and fasta file were made in October 2018.
GC Content60.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEO
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes54
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF197383
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload