Mycobacterium phage RBurden
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| Detailed Information for Phage RBurden | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Evanne Sager, Christina Haight |
| Year Found | 2014 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 47.666935 N, 117.402944 E Map |
| Discovery Notes | Our sample was taken from some soil taken outside of Hughes on Gonzaga University's campus. Our phage grew at room temperature but failed to grow at 37 degrees Celsius. |
| Naming Notes | The excruciatingly long process it took and countless hours it took outside of lab hours to complete the protocols necessary in growing and isolating our phage. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Our phage creates medium sized plaques that are mostly round with rough edges. They were turbid and on average took 3-4 days for plaque growth to be visible. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.4X10^5 |
| Date of SEA Lysate Titering | Mar 27, 2014 |
| Pitt Freezer Box# | 17 |
| Pitt Freezer Box Grid# | D2 |
| Available Files | |
| Restriction Digest Picture | Download |
| EM Picture | Download |
