Gordonia phage RanserRd
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| Detailed Information for Phage RanserRd | |
| Discovery Information | |
| Isolation Host | Gordonia terrae CAG3 |
| Found By | Sophia Benjamin |
| Year Found | 2024 |
| Location Found | Manhattan, KS United States |
| Finding Institution | Kansas State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 12°C |
| GPS Coordinates | 39.200535 N, 96.602767 W Map |
| Discovery Notes | The appearance of my soil was very dark and wet. It also came from a flower pot with the extra nutrients added to the potting mix. Moss and algae were growing on the top, but my soil came from about 3 inches down. I confirmed through many experiments that RanserRd was a bacteriophage of Gordonia terrae, and I also confirmed through lysogen testing that RanserRd is also a bacteriophage of Gordonia lacunea. Through a spot titer, it was found that the titer of RanserRd was 3.3*10^10. This high titer allowed me to do DNA extraction, yielding a 128.2 ng/ul concentration. |
| Naming Notes | The name for this phage came from the street that I live on. Since I got the soil from a flower pot at my house, I thought it would be fun to name the phage after the street it was from. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | According to my lysogeny experiments, RanserRd is a lysogenic phage that can also infect host bacteria Gordonia lacunae. The sensitivity assay showed that RanserRd is not immune to superinfection by two other phages, Archidore and CurlyShirley. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Throughout all of my experiments, my phage grew uniform plaques. They were small, round, and cloudy, signifying that my phage was lysogenic. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 3.3*10^10 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |