Gordonia phage Scootypjr
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Detailed Information for Phage Scootypjr
Discovery Information
Isolation HostGordonia terrae 3612
Former namesNone
Found ByMichael J. Lauer
Year Found2017
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.444034 N, 79.956081 W Map
Discovery NotesThe phage was discovered in a garden pot (in soil) outside of Nordenberg hall on the University of Pittsburgh campus. This pot is right outside of the entrance to the freshman dorms in Nordenberg hall, facing Soldier's and Sailors on the Pitt campus. This pot is white, and contains a large amount of soil in volume. See lab E-notebook for an image.
Naming NotesScootypjr comes from an episode of FOX's show "Futurama"in which they give the main character a "safe" kid's hover-bike that floats above the ground. It's modo is "who's ready for some safe fun?," which is perfect in the case of discovering and characterizing a new bacteriophage. The bike looks like a little puff, similar to what the plaques and actual electron micrograph of the phage turned out to look like.
Sequencing Information
Sequencing Complete?No
Sequencing FacilityPittsburgh Bacteriophage Institute
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Lysogeny NotesLysogen testing has not been conducted to measure host range, however, plaques are very turbid, suggesting lysogenic behavior of the phage. The phage infects Gordonia terrae as a given.
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesPlaques obtained ranged from 1.5 mm to 2.5 mm. They were fairly small and very turbid compared to other phages. The plaques are actually very difficult to see due to their cloudy nature. Many diluted plates contained spots that were very spaced out.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.666x10^11 pff/mL
Date of SEA Lysate Titering Feb 27, 2017
Pitt Freezer Box# 56
Pitt Freezer Box Grid# C10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
Virtual Digest PictureDownload
EM PictureDownload