Mycobacterium phage Pygmy
Pygmy Plaque Picture
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Detailed Information for Phage Pygmy
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByBenjamin M Hughes and Dylan W Stanley
Year Found2023
Location FoundLongview, TX United States
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates32.464444 N, 94.725556 W Map
Discovery NotesPhage Pygmy was isolated from a soil sample collected at 4:35 pm CDT on Tuesday August 22, 2023 from near the edge of the pool at LeTourneau University in Longview, Texas, USA. The location was well covered with green grass. The hole that was dug was 8cm wide and 4cm deep, and 5g of soil from it was ultimately used. The soil was dark, damp and loose. The weather was sunny with a temperature of 39.4°C.
Naming NotesPygmy means very small
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 30, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1762
Genome length (bp)48612
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCCGCCCCCCGA
GC Content66.9%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLeTourneau University
Annotation StatusIn GenBank
Plaque NotesThe plaques looked small and clear (albeit being a temperate phage), with smooth edges and had an average diameter of 1.4 mm (range 0.9 – 2.2 mm, n = 10).
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP758913
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.9 x 10^10 PFU/ml
Pitt Freezer Box# 168
Pitt Freezer Box Grid# B8
Available Files
EM PictureDownload
GenBank File for PhameratorDownload