Mycobacterium phage Shinichiro
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| Detailed Information for Phage Shinichiro | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nicholas Sugimoto |
| Year Found | 2024 |
| Location Found | Bowling Green, KY United States of America |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.98728 N, 86.45204 W Map |
| Discovery Notes | Soil conditions - Very dry and easily broken apart, precipitation was at 0%, collected the sample on the surface of the soil, Doesn’t seem disturbed, there was wildlife such as bunnies roaming nearby during data collection Temperature - 90°F or 32.2°C Location - near the kissing bridge at WKU in Bowling Green KY close to a tree growing on the rocks, near the plants with large and long leaves. Soil had no grass on top but did have grass around. There was barely anything covering the soil where it was collected from. Collected on the very surface of the soil |
| Naming Notes | This name came from my dad who inspired me to go into the biology field. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Using the turbid plaques formed a potential lysogen was plated along with the phage. Once incubated the phage did not create any plaques indicating that there is a lysogen present, and the lysogen has an immunity to bacteriophage Shinichiro. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaque is turbid has a diameter of around 0.35cm, and has no bullseye but a very slight halo can be observed throughout the plaques formed by the phage. Due to the turbid morphology observed this phage lives in a temperate lifestyle. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 2.2 * 10^8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
