Arthrobacter phage SilentRX
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Detailed Information for Phage SilentRX
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByEthan Edwards
Year Found2019
Location FoundManchester, NH United States
Finding InstitutionUniversity of New Hampshire at Manchester
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature18°C
GPS Coordinates42.994046 N, 71.512123 W Map
Discovery NotesLocation Descriptors:

- Backyard of a suburban household
- Area sampled from is was previously a plant grow bed and before that there was a sand pit above


Soil Descriptors:

- Approximately 60% sandy soil and 40% coarse soil
- Slightly moist, as it was in the morning and dew was fresh on the ground
- Flakey
Naming NotesDue to difficulties getting the phage to represent itself on the bacterial lawn (personal error), and the practice of using phage to treat bacterial infections in both animals and humans.
I thought it appropriate to name the phage SilentRX. As it could be administered as "the Silent treatment."
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 22, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1301
Genome length (bp)65232
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length838 bp
GC Content67.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUniversity of New Hampshire at Manchester
Annotation StatusBeing Annotated (Expected completion by 5/1/2020)
Plaque NotesSize (Range): 0.2 mm to 1.2 mm [clear]

Details: 0.1 mm halo [turbid]

Other Notes: Despite range in sizes which only increased after purification, the morphology aside stayed very constant through all stages of processing.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 1.11x10^10 pfu/ml
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload