Arthrobacter phage SilentRX
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Detailed Information for Phage SilentRX
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByEthan Edwards
Year Found2019
Location FoundManchester, NH United States
Finding InstitutionUniversity of New Hampshire at Manchester
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature18°C
GPS Coordinates42.994046 N, 71.512123 W Map
Discovery NotesLocation Descriptors:

- Backyard of a suburban household
- Area sampled from is was previously a plant grow bed and before that there was a sand pit above


Soil Descriptors:

- Approximately 60% sandy soil and 40% coarse soil
- Slightly moist, as it was in the morning and dew was fresh on the ground
- Flakey
Naming NotesDue to difficulties getting the phage to represent itself on the bacterial lawn (personal error), and the practice of using phage to treat bacterial infections in both animals and humans.
I thought it appropriate to name the phage SilentRX. As it could be administered as "the Silent treatment."
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 22, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1301
Genome length (bp)65232
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length838 bp
GC Content67.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAP
SubclusterAP4
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of New Hampshire at Manchester
Annotation StatusIn GenBank
Plaque NotesSize (Range): 0.2 mm to 1.2 mm [clear]

Details: 0.1 mm halo [turbid]

Other Notes: Despite range in sizes which only increased after purification, the morphology aside stayed very constant through all stages of processing.
Has been Phamerated?Yes
Gene List

Click to View

SilentRX_1

SilentRX_2

SilentRX_3

SilentRX_4

SilentRX_5

SilentRX_6

SilentRX_7

SilentRX_8

SilentRX_9

SilentRX_10
(ParB-like nuclease domain protein)

SilentRX_11
(DNA methylase)

SilentRX_12
(terminase small subunit)

SilentRX_13
(DNA binding protein)

SilentRX_14

SilentRX_15
(DNA methylase)

SilentRX_16
(terminase large subunit)

SilentRX_17

SilentRX_18
(portal protein)

SilentRX_19
(capsid maturation protease and MuF-like fusion protein)

SilentRX_20

SilentRX_21
(major capsid hexamer protein)

SilentRX_22

SilentRX_23

SilentRX_24

SilentRX_25
(endolysin)

SilentRX_26
(head-to-tail adaptor)

SilentRX_27

SilentRX_28

SilentRX_29
(tail assembly protein)

SilentRX_30
(tail assembly chaperone)

SilentRX_31
(tape measure protein)

SilentRX_32
(minor tail protein)

SilentRX_33
(minor tail protein)

SilentRX_34

SilentRX_35
(minor tail protein)

SilentRX_36

SilentRX_37

SilentRX_38

SilentRX_39

SilentRX_40

SilentRX_41
(RusA-like resolvase)

SilentRX_42

SilentRX_43

SilentRX_44

SilentRX_45

SilentRX_46
(lipoprotein)

SilentRX_47

SilentRX_48

SilentRX_49

SilentRX_50

SilentRX_51

SilentRX_52

SilentRX_53

SilentRX_54

SilentRX_55
(HicA-like toxin)

SilentRX_56

SilentRX_57

SilentRX_58

SilentRX_59
(DNA recombinase)

SilentRX_60

SilentRX_61
(deoxyuridine triphosphatase)

SilentRX_62
(HNH endonuclease)

SilentRX_63

SilentRX_64
(DNA binding protein)

SilentRX_65

SilentRX_66

SilentRX_67
(DNA primase/helicase)

SilentRX_68
(RepA-like replication initiator)

SilentRX_69

SilentRX_70
(ssDNA binding protein)

SilentRX_71

SilentRX_72

SilentRX_73

SilentRX_74

SilentRX_75

SilentRX_76

SilentRX_77

SilentRX_78

SilentRX_79
(glycosyltransferase)

SilentRX_80

SilentRX_81

SilentRX_82

SilentRX_83

SilentRX_84

SilentRX_85

SilentRX_86

SilentRX_87

SilentRX_88
(DNA primase domain protein)

SilentRX_89

SilentRX_90

SilentRX_91

SilentRX_92

SilentRX_93

SilentRX_94

SilentRX_95

SilentRX_96

SilentRX_97

SilentRX_98

SilentRX_99

SilentRX_100

SilentRX_101

SilentRX_102

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW862992
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.11x10^10 pfu/ml
Pitt Freezer Box# 116
Pitt Freezer Box Grid# B10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload