Mycobacterium phage Sparky
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Detailed Information for Phage Sparky
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKassie Simmons-Yager
Year Found2009
Location FoundCorvallis, OR USA
Finding InstitutionOregon State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates44.562919 N, 123.274203 W Map
Discovery NotesMoist dark top soil under drain drip spot. Same location and enrichment as 2009ORSUdarthphagerKT
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 25, 2011
Genome length (bp)63334
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGATGGCAT
GC Content65.2%
Fasta file available?Yes: Download fasta file
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesClear plaques, 1/4 mm in diameter grows in 24hrs incubation temperature
Has been Phamerated?Yes
Gene List

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Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKM083128
Refseq NumberNC_026597
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2009ORSUsparkyKSY
SEA Lysate Titer 5.8e9
Date of SEA Lysate Titering Nov 13, 2009
Pitt Freezer Box# 5
Pitt Freezer Box Grid# 51
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload